Detailed information of TA system TA089956

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4988319..4989154
• Replicon: chromosome
• Accession: NZ_CP025251
• Organism: Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: BH100B_RS25300
• Protein ID: WP_001094426.1
• Coordinates: 4988777..4989154 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: BH100B_RS25295
• Protein ID: WP_001285575.1
• Coordinates: 4988319..4988687 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100B_RS25260	4984211..4984891	+	681	WP_001282919.1	WYL domain-containing protein	-
BH100B_RS25265	4985039..4985716	+	678	WP_001097301.1	hypothetical protein	-
BH100B_RS25270	4985722..4985955	+	234	WP_001278290.1	DUF905 family protein	-
BH100B_RS25275	4986045..4986863	+	819	WP_065377599.1	DUF945 domain-containing protein	-
BH100B_RS25280	4986955..4987440	+	486	WP_000214415.1	antirestriction protein	-
BH100B_RS25285	4987455..4987931	+	477	WP_001298074.1	RadC family protein	-
BH100B_RS25290	4988018..4988239	+	222	WP_000692350.1	DUF987 domain-containing protein	-
BH100B_RS25295	4988319..4988687	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BH100B_RS25300	4988777..4989154	+	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
BH100B_RS25305	4989151..4989639	+	489	WP_000761683.1	hypothetical protein	-
BH100B_RS25310	4989651..4989848	+	198	WP_000839272.1	DUF957 domain-containing protein	-
BH100B_RS27440	4989927..4990085	+	159	WP_001467148.1	hypothetical protein	-
BH100B_RS25320	4991033..4993069	+	2037	WP_000417001.1	hypothetical protein	-
BH100B_RS25325	4993468..4993647	-	180	WP_001298063.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T89956 WP_001094426.1 NZ_CP025251:4988777-4989154 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T89956 NZ_CP025251:4988777-4989154 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT89956 WP_001285575.1 NZ_CP025251:4988319-4988687 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT89956 NZ_CP025251:4988319-4988687 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5