Detailed information of TA system TA089950

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3337310..3338144
• Replicon: chromosome
• Accession: NZ_CP025251
• Organism: Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A838AQY5
• Locus tag: BH100B_RS16940
• Protein ID: WP_023152077.1
• Coordinates: 3337767..3338144 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BH100B_RS16935
• Protein ID: WP_065377585.1
• Coordinates: 3337310..3337678 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100B_RS16900	3334125..3334580	+	456	WP_000581504.1	hypothetical protein	-
BH100B_RS16905	3334659..3334892	+	234	WP_001119729.1	DUF905 family protein	-
BH100B_RS16915	3334992..3335810	+	819	WP_032250973.1	DUF945 domain-containing protein	-
BH100B_RS16920	3335892..3336371	+	480	WP_023568406.1	antirestriction protein	-
BH100B_RS16925	3336387..3336863	+	477	WP_001186773.1	RadC family protein	-
BH100B_RS16930	3336926..3337147	+	222	WP_000692305.1	DUF987 domain-containing protein	-
BH100B_RS16935	3337310..3337678	+	369	WP_065377585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
BH100B_RS16940	3337767..3338144	+	378	WP_023152077.1	type IV toxin-antitoxin system toxin CbtA	Toxin
BH100B_RS16945	3338141..3338629	+	489	WP_065377586.1	hypothetical protein	-
BH100B_RS16950	3338641..3338838	+	198	WP_000839281.1	DUF957 domain-containing protein	-
BH100B_RS16955	3338935..3339444	+	510	WP_050582268.1	DUF4942 domain-containing protein	-
BH100B_RS16960	3339847..3340017	+	171	Protein_3103	IS110 family transposase	-
BH100B_RS16965	3340816..3341799	+	984	WP_001331698.1	KpsF/GutQ family sugar-phosphate isomerase	-
BH100B_RS16970	3341871..3343019	+	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3339913..3340017	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14056.12 Da
• Isoelectric Point: 7.8839

>T89950 WP_023152077.1 NZ_CP025251:3337767-3338144 [Escherichia coli]
MKTLPVLPGQVASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRAIGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T89950 NZ_CP025251:3337767-3338144 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGTGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAATCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13580.37 Da
• Isoelectric Point: 5.0580

>AT89950 WP_065377585.1 NZ_CP025251:3337310-3337678 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT89950 NZ_CP025251:3337310-3337678 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A838AQY5