Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2182236..2183067 Replicon chromosome
Accession NZ_CP025251
Organism Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag BH100B_RS11260 Protein ID WP_000854814.1
Coordinates 2182693..2183067 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID -
Locus tag BH100B_RS11255 Protein ID WP_024174331.1
Coordinates 2182236..2182604 (+) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH100B_RS11215 2177293..2178363 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
BH100B_RS11220 2178499..2179176 + 678 WP_001362823.1 hypothetical protein -
BH100B_RS11225 2179192..2179602 + 411 WP_000846704.1 hypothetical protein -
BH100B_RS11235 2179823..2180644 + 822 WP_001234571.1 DUF945 domain-containing protein -
BH100B_RS11240 2180907..2181380 + 474 WP_000855074.1 antirestriction protein -
BH100B_RS11245 2181396..2181872 + 477 WP_001541535.1 RadC family protein -
BH100B_RS11250 2181941..2182162 + 222 WP_000692346.1 DUF987 domain-containing protein -
BH100B_RS11255 2182236..2182604 + 369 WP_024174331.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
BH100B_RS11260 2182693..2183067 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
BH100B_RS11265 2183064..2183258 + 195 WP_000988601.1 hypothetical protein -
BH100B_RS27400 2183271..2183384 + 114 WP_001161660.1 DUF957 domain-containing protein -
BH100B_RS11270 2183673..2183819 - 147 Protein_2054 transposase domain-containing protein -
BH100B_RS11275 2183873..2184055 + 183 WP_001016348.1 hypothetical protein -
BH100B_RS11280 2184156..2184485 - 330 WP_000450409.1 DUF496 family protein -
BH100B_RS11285 2184657..2185715 - 1059 WP_001200890.1 FUSC family protein -
BH100B_RS11290 2185913..2186386 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
BH100B_RS11295 2186505..2187671 - 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(19-117)


Sequences


Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T89946 WP_000854814.1 NZ_CP025251:2182693-2183067 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T89946 NZ_CP025251:2182693-2183067 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13603.42 Da        Isoelectric Point: 6.7386

>AT89946 WP_024174331.1 NZ_CP025251:2182236-2182604 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Download         Length: 369 bp

>AT89946 NZ_CP025251:2182236-2182604 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829FY50


Antitoxin

Source ID Structure

References