Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 572204..572822 Replicon chromosome
Accession NZ_CP025251
Organism Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BH100B_RS02880 Protein ID WP_001291435.1
Coordinates 572204..572422 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BH100B_RS02885 Protein ID WP_000344800.1
Coordinates 572448..572822 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH100B_RS02845 567491..568063 + 573 WP_000779830.1 YbaY family lipoprotein -
BH100B_RS02850 568094..568405 - 312 WP_000409908.1 MGMT family protein -
BH100B_RS02860 568784..569137 + 354 WP_000878138.1 DUF1428 family protein -
BH100B_RS02865 569179..570729 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BH100B_RS02870 570893..571363 - 471 WP_000136192.1 YlaC family protein -
BH100B_RS02875 571479..572030 - 552 WP_000102539.1 maltose O-acetyltransferase -
BH100B_RS02880 572204..572422 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BH100B_RS02885 572448..572822 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BH100B_RS02890 573367..576517 - 3151 Protein_524 multidrug efflux RND transporter permease subunit -
BH100B_RS02895 576540..577733 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T89934 WP_001291435.1 NZ_CP025251:c572422-572204 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T89934 NZ_CP025251:c572422-572204 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT89934 WP_000344800.1 NZ_CP025251:c572822-572448 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT89934 NZ_CP025251:c572822-572448 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References