Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 60568..61118 Replicon chromosome
Accession NZ_CP025251
Organism Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag BH100B_RS00320 Protein ID WP_001160966.1
Coordinates 60804..61118 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag BH100B_RS00315 Protein ID WP_000125568.1
Coordinates 60568..60801 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH100B_RS00290 55641..55928 + 288 WP_000203741.1 ferredoxin-like protein FixX -
BH100B_RS00295 55987..57318 + 1332 WP_001183198.1 MFS transporter -
BH100B_RS00300 57426..57956 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
BH100B_RS00305 57949..59811 + 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
BH100B_RS00310 60003..60482 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
BH100B_RS00315 60568..60801 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
BH100B_RS00320 60804..61118 + 315 WP_001160966.1 CcdB family protein Toxin
BH100B_RS00325 61115..61963 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
BH100B_RS00330 61970..62347 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
BH100B_RS00335 62350..63171 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
BH100B_RS00340 63168..64157 - 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
BH100B_RS00345 64157..65443 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T89931 WP_001160966.1 NZ_CP025251:60804-61118 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T89931 NZ_CP025251:60804-61118 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT89931 WP_000125568.1 NZ_CP025251:60568-60801 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT89931 NZ_CP025251:60568-60801 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References