Detailed information of TA system TA089915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 388468..389123
• Replicon: chromosome
• Accession: NZ_CP025228
• Organism: Actinomyces qiguomingii strain actinomyces sp. 410

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CWT10_RS01620
• Protein ID: WP_103063761.1
• Coordinates: 388704..389123 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CWT10_RS01615
• Protein ID: WP_103063762.1
• Coordinates: 388468..388707 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWT10_RS01595	383878..384204	+	327	WP_103063782.1	pilus assembly protein TadE	-
CWT10_RS01600	384201..384587	+	387	WP_103063764.1	flp pilus-assembly TadE/G-like family protein	-
CWT10_RS01605	384752..385522	+	771	WP_103063781.1	A24 family peptidase	-
CWT10_RS01610	385711..388248	-	2538	WP_103063763.1	DUF1998 domain-containing protein	-
CWT10_RS01615	388468..388707	+	240	WP_103063762.1	Arc family DNA-binding protein	Antitoxin
CWT10_RS01620	388704..389123	+	420	WP_103063761.1	type II toxin-antitoxin system VapC family toxin	Toxin
CWT10_RS01625	389171..389467	+	297	WP_128683226.1	hypothetical protein	-
CWT10_RS01630	389531..389824	+	294	WP_103063759.1	hypothetical protein	-
CWT10_RS01635	390967..391812	+	846	WP_158247680.1	alpha/beta hydrolase	-
CWT10_RS01640	391992..392273	-	282	WP_103063757.1	putative addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-127)

Antitoxin

• Arc-like DNA binding domain (9-39)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15281.64 Da
• Isoelectric Point: 5.8701

>T89915 WP_103063761.1 NZ_CP025228:388704-389123 [Actinomyces qiguomingii]
VIILDTNVISEPLRSTPEPAVVEWLDRQPQESLFVTALTLAELRRGVLALPEGRGRDTLQYRLEETLLPFFGDRILPFNG
AAASAWAQLQARATGTGRPLPVMDSLIGAIAYAHSMTLATRNGRDFKPMGLKLIDPWTA

Nucleotide

• Length: 420 bp

>T89915 NZ_CP025228:388704-389123 [Actinomyces qiguomingii]
GTGATCATTCTCGATACGAATGTCATCTCCGAGCCGTTGCGGAGCACACCGGAGCCGGCGGTTGTTGAATGGCTCGATCG
GCAGCCTCAGGAGTCGCTGTTCGTCACCGCCCTGACGCTTGCCGAGTTGCGGCGGGGTGTTTTGGCCCTGCCGGAGGGGC
GAGGACGAGACACGCTCCAGTACCGATTGGAGGAGACGCTACTGCCGTTCTTCGGCGACAGGATCCTTCCGTTCAATGGT
GCTGCCGCAAGCGCCTGGGCGCAGTTGCAGGCGCGGGCAACCGGAACAGGGCGCCCCCTGCCGGTGATGGACTCCCTTAT
CGGGGCAATCGCGTATGCCCACTCGATGACACTGGCCACGCGGAATGGGCGCGACTTCAAGCCAATGGGATTGAAACTCA
TCGATCCGTGGACGGCATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8512.62 Da
• Isoelectric Point: 5.0484

>AT89915 WP_103063762.1 NZ_CP025228:388468-388707 [Actinomyces qiguomingii]
MADVLVRNLPDETHRALKALAKSHGRSMEAEIRQILGRAVAANEHPGLGSLLTSIRDELGPVELDVDRDGSPYAPVDLS

Nucleotide

• Length: 240 bp

>AT89915 NZ_CP025228:388468-388707 [Actinomyces qiguomingii]
ATGGCGGATGTTCTGGTTCGGAACCTGCCCGATGAGACCCATCGGGCACTGAAGGCGCTGGCGAAGTCACATGGTCGCAG
CATGGAGGCCGAGATTCGGCAGATCCTGGGACGGGCTGTGGCCGCGAATGAACACCCCGGCCTTGGGTCGCTGCTGACTT
CAATACGCGATGAGCTTGGGCCTGTGGAACTCGACGTGGATCGTGACGGCAGTCCCTATGCCCCGGTGGATCTGTCGTGA