Detailed information of TA system TA089914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-RelB
• Location: 2425002..2425524
• Replicon: chromosome
• Accession: NZ_CP025227
• Organism: Actinomyces wuliandei strain 299

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CWS50_RS10040
• Protein ID: WP_127842683.1
• Coordinates: 2425261..2425524 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: CWS50_RS10035
• Protein ID: WP_127842682.1
• Coordinates: 2425002..2425259 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWS50_RS10025	2420826..2421926	-	1101	WP_127842680.1	AAA family ATPase	-
CWS50_RS10030	2422004..2424712	-	2709	WP_127842681.1	pyruvate, phosphate dikinase	-
CWS50_RS10035	2425002..2425259	+	258	WP_127842682.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CWS50_RS10040	2425261..2425524	+	264	WP_127842683.1	Txe/YoeB family addiction module toxin	Toxin
CWS50_RS10045	2425567..2426145	-	579	WP_127842684.1	hypothetical protein	-
CWS50_RS10050	2426135..2426929	-	795	WP_127842685.1	hypothetical protein	-
CWS50_RS10055	2426884..2427270	-	387	WP_127842686.1	hypothetical protein	-
CWS50_RS10060	2427394..2428473	-	1080	WP_127842687.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10737.02 Da
• Isoelectric Point: 9.4176

>T89914 WP_127842683.1 NZ_CP025227:2425261-2425524 [Actinomyces wuliandei]
MAVAWDNDAWEDYLWWQKQDRKTLRRINQLLRDIQRDATTGTGRPEPLRYNLAGYWSRRITSEHRLVYRVDERGDIHVLS
CRYHYER

Nucleotide

• Length: 264 bp

>T89914 NZ_CP025227:2425261-2425524 [Actinomyces wuliandei]
ATGGCGGTCGCATGGGACAACGACGCCTGGGAGGACTACCTCTGGTGGCAGAAGCAGGACCGTAAGACCCTCAGACGCAT
CAACCAGCTCCTCCGCGACATCCAGCGCGACGCGACCACCGGGACCGGCAGACCCGAGCCCCTGCGCTACAACCTGGCCG
GGTACTGGTCGCGCCGCATCACCAGCGAGCACCGCCTTGTCTACCGTGTGGACGAGCGAGGAGACATCCACGTGCTCTCA
TGCCGGTACCACTACGAGCGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9593.70 Da
• Isoelectric Point: 4.4901

>AT89914 WP_127842682.1 NZ_CP025227:2425002-2425259 [Actinomyces wuliandei]
MRTMTYTESRARYAEVLDSVVNDCEEVVITRAGHEPAVIVSLAEYQSLKETDYLLRNPANARHLINAIARSEAGQDAYHD
LIEVE

Nucleotide

• Length: 258 bp

>AT89914 NZ_CP025227:2425002-2425259 [Actinomyces wuliandei]
ATGAGGACCATGACCTACACCGAATCGCGCGCCCGCTACGCCGAGGTCCTTGACTCCGTGGTCAACGACTGCGAGGAGGT
CGTCATCACCCGAGCAGGCCACGAGCCCGCCGTCATCGTCTCCCTGGCCGAGTACCAGTCCCTCAAGGAGACCGACTACC
TCCTGCGCAACCCGGCCAACGCCCGGCACCTGATCAACGCGATCGCGCGCTCCGAGGCCGGGCAGGACGCCTACCACGAC
CTCATTGAGGTCGAATGA