Detailed information of TA system TA089913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3321334..3321970
• Replicon: chromosome
• Accession: NZ_CP025226
• Organism: Bacillus licheniformis strain PB3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M5P3Q9
• Locus tag: CXG95_RS17460
• Protein ID: WP_003179128.1
• Coordinates: 3321334..3321684 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M5PDU2
• Locus tag: CXG95_RS17465
• Protein ID: WP_006638778.1
• Coordinates: 3321689..3321970 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXG95_RS17420	3316375..3316974	-	600	WP_003179145.1	SpoIIE family protein phosphatase	-
CXG95_RS17425	3316971..3317762	-	792	WP_003179144.1	RNA polymerase sigma factor SigB	-
CXG95_RS17430	3317728..3318213	-	486	WP_003179142.1	anti-sigma B factor RsbW	-
CXG95_RS17435	3318213..3318539	-	327	WP_003179140.1	anti-sigma factor antagonist	-
CXG95_RS17440	3318598..3319605	-	1008	WP_003179137.1	PP2C family protein-serine/threonine phosphatase	-
CXG95_RS17445	3319616..3320017	-	402	WP_003179135.1	anti-sigma regulatory factor	-
CXG95_RS17450	3320020..3320385	-	366	WP_003179132.1	STAS domain-containing protein	-
CXG95_RS17455	3320389..3321216	-	828	WP_003179130.1	RsbT co-antagonist protein RsbRA	-
CXG95_RS17460	3321334..3321684	-	351	WP_003179128.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
CXG95_RS17465	3321689..3321970	-	282	WP_006638778.1	hypothetical protein	Antitoxin
CXG95_RS17470	3322082..3323251	-	1170	WP_026080763.1	alanine racemase	-
CXG95_RS17475	3323476..3324435	-	960	WP_152847888.1	outer membrane lipoprotein carrier protein LolA	-
CXG95_RS17480	3324778..3325377	+	600	WP_003179118.1	rhomboid family intramembrane serine protease	-
CXG95_RS17485	3325374..3326855	-	1482	WP_009330132.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12990.03 Da
• Isoelectric Point: 5.7234

>T89913 WP_003179128.1 NZ_CP025226:c3321684-3321334 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Nucleotide

• Length: 351 bp

>T89913 NZ_CP025226:c3321684-3321334 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10649.03 Da
• Isoelectric Point: 4.6920

>AT89913 WP_006638778.1 NZ_CP025226:c3321970-3321689 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT89913 NZ_CP025226:c3321970-3321689 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A6I7FHI4

Antitoxin

• AlphaFold DB: M5PDU2