Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3321334..3321970 Replicon chromosome
Accession NZ_CP025226
Organism Bacillus licheniformis strain PB3

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag CXG95_RS17460 Protein ID WP_003179128.1
Coordinates 3321334..3321684 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag CXG95_RS17465 Protein ID WP_006638778.1
Coordinates 3321689..3321970 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXG95_RS17420 3316375..3316974 - 600 WP_003179145.1 SpoIIE family protein phosphatase -
CXG95_RS17425 3316971..3317762 - 792 WP_003179144.1 RNA polymerase sigma factor SigB -
CXG95_RS17430 3317728..3318213 - 486 WP_003179142.1 anti-sigma B factor RsbW -
CXG95_RS17435 3318213..3318539 - 327 WP_003179140.1 anti-sigma factor antagonist -
CXG95_RS17440 3318598..3319605 - 1008 WP_003179137.1 PP2C family protein-serine/threonine phosphatase -
CXG95_RS17445 3319616..3320017 - 402 WP_003179135.1 anti-sigma regulatory factor -
CXG95_RS17450 3320020..3320385 - 366 WP_003179132.1 STAS domain-containing protein -
CXG95_RS17455 3320389..3321216 - 828 WP_003179130.1 RsbT co-antagonist protein RsbRA -
CXG95_RS17460 3321334..3321684 - 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CXG95_RS17465 3321689..3321970 - 282 WP_006638778.1 hypothetical protein Antitoxin
CXG95_RS17470 3322082..3323251 - 1170 WP_026080763.1 alanine racemase -
CXG95_RS17475 3323476..3324435 - 960 WP_152847888.1 outer membrane lipoprotein carrier protein LolA -
CXG95_RS17480 3324778..3325377 + 600 WP_003179118.1 rhomboid family intramembrane serine protease -
CXG95_RS17485 3325374..3326855 - 1482 WP_009330132.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T89913 WP_003179128.1 NZ_CP025226:c3321684-3321334 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T89913 NZ_CP025226:c3321684-3321334 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT89913 WP_006638778.1 NZ_CP025226:c3321970-3321689 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT89913 NZ_CP025226:c3321970-3321689 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References