Detailed information of TA system TA089910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 4074054..4074848
• Replicon: chromosome
• Accession: NZ_CP025225
• Organism: Enterobacter cancerogenus strain CR-Eb1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: CWI88_RS19365
• Protein ID: WP_101738295.1
• Coordinates: 4074327..4074848 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: D2ZA22
• Locus tag: CWI88_RS19360
• Protein ID: WP_006174147.1
• Coordinates: 4074054..4074323 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI88_RS19340	4069844..4071142	+	1299	WP_101738294.1	DUF445 domain-containing protein	-
CWI88_RS19345	4071112..4072188	-	1077	WP_006174141.1	DUF2955 domain-containing protein	-
CWI88_RS19350	4072178..4073245	-	1068	WP_088208796.1	HlyD family secretion protein	-
CWI88_RS19355	4073242..4073712	-	471	WP_058609602.1	MarR family transcriptional regulator	-
CWI88_RS19360	4074054..4074323	+	270	WP_006174147.1	DUF1778 domain-containing protein	Antitoxin
CWI88_RS19365	4074327..4074848	+	522	WP_101738295.1	GNAT family N-acetyltransferase	Toxin
CWI88_RS19370	4074901..4076316	-	1416	WP_101738296.1	aldehyde dehydrogenase family protein	-
CWI88_RS19375	4076417..4077319	+	903	WP_006174153.1	LysR family transcriptional regulator	-
CWI88_RS19380	4077306..4077785	-	480	WP_006174154.1	carboxymuconolactone decarboxylase family protein	-
CWI88_RS19385	4077887..4079275	+	1389	WP_101738297.1	PLP-dependent aminotransferase family protein	-
CWI88_RS19390	4079360..4079521	-	162	WP_058609595.1	DUF1127 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19882.78 Da
• Isoelectric Point: 6.8652

>T89910 WP_101738295.1 NZ_CP025225:4074327-4074848 [Enterobacter cancerogenus]
VNDVKIGIFSEDVEYELSQFDCGEVSLNTFLAEHLKRQHREKFLRAYVLTTREENPRVLGYYTLSGSCFEKAYLPSKTQQ
KRIPYKNVPSVTLGRLAIDKRIQGQGFGELLVTHAMKTVYLASFAVDIHGLFVEAQNDKARNFYLKLGFIPLLAENEFTL
FIPTKTFESVFEE

Nucleotide

• Length: 522 bp

>T89910 NZ_CP025225:4074327-4074848 [Enterobacter cancerogenus]
GTGAACGATGTGAAGATAGGGATTTTTTCAGAAGACGTTGAGTACGAACTGAGCCAGTTCGATTGCGGAGAAGTAAGCCT
TAACACCTTCCTTGCGGAACACCTTAAACGCCAGCATCGCGAAAAGTTTTTGCGTGCCTACGTGCTCACGACCCGCGAGG
AAAACCCGCGAGTTCTGGGTTATTACACGCTCTCCGGGAGTTGTTTTGAAAAAGCTTACCTGCCCTCTAAAACTCAGCAG
AAGCGCATTCCTTATAAAAATGTGCCGAGCGTAACCTTGGGGCGTCTGGCTATTGATAAACGCATTCAGGGGCAAGGTTT
TGGTGAGTTACTTGTCACCCATGCTATGAAGACCGTCTATTTGGCCTCCTTTGCCGTCGACATTCATGGTCTATTTGTTG
AAGCGCAGAACGATAAAGCCAGAAATTTTTACCTCAAACTGGGGTTCATACCGCTTCTGGCCGAGAATGAATTCACGCTC
TTCATACCGACGAAAACCTTTGAAAGCGTGTTTGAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10142.57 Da
• Isoelectric Point: 7.0164

>AT89910 WP_006174147.1 NZ_CP025225:4074054-4074323 [Enterobacter cancerogenus]
MTALKKQRIDLRLSEDDKSLIEEAAAMTNQSISQFMVSTASERAAEVIDRHRRMILNEASWNTVMEAISNPPAPNDRLKR
AAKRLQDME

Nucleotide

• Length: 270 bp

>AT89910 NZ_CP025225:4074054-4074323 [Enterobacter cancerogenus]
ATGACAGCACTGAAGAAACAGCGCATTGATCTGAGGTTAAGCGAAGACGATAAAAGCCTGATCGAAGAAGCTGCAGCAAT
GACCAACCAGTCAATTTCACAGTTCATGGTCAGCACGGCTTCTGAACGCGCTGCGGAAGTTATCGATCGACACCGTCGCA
TGATCCTTAATGAAGCCTCCTGGAATACGGTAATGGAGGCCATCAGCAACCCGCCGGCACCAAATGACCGCCTGAAACGC
GCGGCGAAGCGTCTCCAGGATATGGAGTAA

Structures

Antitoxin

• AlphaFold DB: D2ZA22