Detailed information of TA system TA089909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3592169..3592789
• Replicon: chromosome
• Accession: NZ_CP025225
• Organism: Enterobacter cancerogenus strain CR-Eb1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2ZG55
• Locus tag: CWI88_RS17105
• Protein ID: WP_006176938.1
• Coordinates: 3592571..3592789 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: D2ZG56
• Locus tag: CWI88_RS17100
• Protein ID: WP_006176939.1
• Coordinates: 3592169..3592543 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI88_RS17090	3587296..3588489	+	1194	WP_006176941.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
CWI88_RS17095	3588512..3591658	+	3147	WP_058609279.1	multidrug efflux RND transporter permease subunit AcrB	-
CWI88_RS17100	3592169..3592543	+	375	WP_006176939.1	Hha toxicity modulator TomB	Antitoxin
CWI88_RS17105	3592571..3592789	+	219	WP_006176938.1	hemolysin expression modulator Hha	Toxin
CWI88_RS17110	3592995..3593546	+	552	WP_088207831.1	maltose O-acetyltransferase	-
CWI88_RS17115	3593663..3594151	+	489	WP_042320836.1	membrane protein	-
CWI88_RS17120	3594102..3595562	-	1461	WP_101738140.1	PLP-dependent aminotransferase family protein	-
CWI88_RS17125	3595664..3596374	+	711	WP_101738607.1	GNAT family N-acetyltransferase	-
CWI88_RS17130	3596371..3596511	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
CWI88_RS17135	3596514..3596774	-	261	WP_006176933.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T89909 WP_006176938.1 NZ_CP025225:3592571-3592789 [Enterobacter cancerogenus]
MSEKPLTKIDYLMRLRRCQSLDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T89909 NZ_CP025225:3592571-3592789 [Enterobacter cancerogenus]
ATGTCTGAAAAACCATTAACCAAGATCGATTATTTGATGCGCTTGCGACGTTGTCAGTCACTGGACACGCTTGAACGTGT
TATCGAAAAAAATAAATATGAGCTTTCTGATAACGAACTGGCTGTATTTTACTCAGCAGCGGATCATCGCCTGGCCGAGT
TAACCATGAATAAGCTTTACGACAAAATACCGTCTTCCGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14467.25 Da
• Isoelectric Point: 4.8886

>AT89909 WP_006176939.1 NZ_CP025225:3592169-3592543 [Enterobacter cancerogenus]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFINASRANPVSLSC

Nucleotide

• Length: 375 bp

>AT89909 NZ_CP025225:3592169-3592543 [Enterobacter cancerogenus]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGTCTTGC
CAATCTTGATGAAAGTAACCATGGGTGGGTTAACGACCCCACCTCGGCCATCAATTTGCAGCTTAATGAGCTGATAGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGATGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAATCGGCT
ATTCCGTTGTTTCATCAATGCAAGTCGAGCTAATCCTGTTAGCCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: D2ZG55

Antitoxin

• AlphaFold DB: D2ZG56