Detailed information of TA system TA089896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 55448..56109
• Replicon: chromosome
• Accession: NZ_CP025225
• Organism: Enterobacter cancerogenus strain CR-Eb1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D2ZIF7
• Locus tag: CWI88_RS00295
• Protein ID: WP_006177650.1
• Coordinates: 55711..56109 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CWI88_RS00290
• Protein ID: WP_101736741.1
• Coordinates: 55448..55714 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI88_RS00265	50665..51786	-	1122	WP_101736737.1	efflux RND transporter periplasmic adaptor subunit	-
CWI88_RS00270	51934..52503	-	570	WP_088208957.1	TetR/AcrR family transcriptional regulator	-
CWI88_RS00275	52686..53105	+	420	WP_101736738.1	GNAT family N-acetyltransferase	-
CWI88_RS00280	53102..53749	-	648	WP_101736739.1	TetR/AcrR family transcriptional regulator	-
CWI88_RS00285	53843..55336	+	1494	WP_101736740.1	MFS transporter	-
CWI88_RS00290	55448..55714	+	267	WP_101736741.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CWI88_RS00295	55711..56109	+	399	WP_006177650.1	type II toxin-antitoxin system VapC family toxin	Toxin
CWI88_RS00300	56244..57044	+	801	WP_101736742.1	lipoprotein NlpA	-
CWI88_RS00305	57050..57730	-	681	WP_101736743.1	EAL domain-containing protein	-
CWI88_RS00310	57807..58427	-	621	WP_042321296.1	response regulator transcription factor	-
CWI88_RS00315	58999..59634	+	636	WP_042321474.1	response regulator transcription factor	-
CWI88_RS00320	59675..60739	-	1065	WP_101736744.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14606.72 Da
• Isoelectric Point: 5.9718

>T89896 WP_006177650.1 NZ_CP025225:55711-56109 [Enterobacter cancerogenus]
MRHMLDTNIVSHLFKRHPGVINRMACLTPDDVCISSITEAELLYGADKKQSERLKKTIQAFLSTITVCDWDSEAAATYGA
LRAAMEKRGNVMGDLDQLIAAHAISQGTTIVTNDRAFLMVQELSVEDWTHAA

Nucleotide

• Length: 399 bp

>T89896 NZ_CP025225:55711-56109 [Enterobacter cancerogenus]
ATGAGGCATATGCTGGATACGAATATTGTGAGTCATCTGTTTAAAAGGCACCCAGGCGTAATAAACAGAATGGCTTGCCT
GACACCGGACGACGTTTGCATATCCAGTATTACAGAAGCAGAACTGCTCTACGGTGCCGATAAAAAACAAAGCGAGAGGC
TGAAAAAAACAATCCAGGCGTTTCTGAGCACCATCACCGTCTGCGACTGGGACAGCGAGGCCGCAGCCACCTACGGCGCG
CTCCGTGCGGCGATGGAAAAGAGAGGCAACGTCATGGGCGATCTCGACCAGCTGATCGCTGCGCATGCCATCAGCCAGGG
CACCACCATTGTCACGAACGATCGTGCCTTTCTGATGGTGCAGGAGTTAAGCGTGGAAGACTGGACGCACGCTGCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10212.38 Da
• Isoelectric Point: 6.3656

>AT89896 WP_101736741.1 NZ_CP025225:55448-55714 [Enterobacter cancerogenus]
MERKAKLFKRGRSQAVMLPAEFTFDAESVYIRRDDDGNVVLSEKSASTRNWDNFFRLLENTSVPENFLSKEERNQSITRR
DPLDGVQG

Nucleotide

• Length: 267 bp

>AT89896 NZ_CP025225:55448-55714 [Enterobacter cancerogenus]
ATGGAACGCAAAGCAAAACTGTTTAAAAGAGGGCGAAGCCAGGCGGTAATGCTGCCTGCTGAATTCACGTTTGATGCGGA
GAGTGTTTATATCCGGCGGGATGACGACGGGAATGTGGTGCTGTCGGAGAAGTCCGCCAGCACCAGGAACTGGGATAATT
TTTTCAGACTGCTGGAAAACACATCTGTACCTGAAAACTTTTTGAGTAAGGAGGAGAGAAACCAGAGCATAACCAGAAGA
GACCCGCTGGATGGAGTTCAGGGATGA

Structures

Toxin

• AlphaFold DB: D2ZIF7