Detailed information of TA system TA089892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 70390..71033
• Replicon: plasmid unnamed2
• Accession: NZ_CP025213
• Organism: Klebsiella pneumoniae strain HZW25

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CW753_RS27430
• Protein ID: WP_048295728.1
• Coordinates: 70390..70806 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: CW753_RS27435
• Protein ID: WP_001261276.1
• Coordinates: 70803..71033 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CW753_RS27400 (CW753_27590)	65610..66044	-	435	WP_001567367.1	cell envelope integrity protein TolA	-
CW753_RS27405 (CW753_27595)	66091..66639	-	549	WP_001567366.1	thioredoxin fold domain-containing protein	-
CW753_RS27415 (CW753_27605)	67091..67441	+	351	WP_167878626.1	hypothetical protein	-
CW753_RS30205	67541..67663	+	123	WP_256046161.1	hypothetical protein	-
CW753_RS27425 (CW753_27615)	68082..70220	+	2139	WP_048295729.1	AAA family ATPase	-
CW753_RS27430 (CW753_27620)	70390..70806	-	417	WP_048295728.1	type II toxin-antitoxin system VapC family toxin	Toxin
CW753_RS27435 (CW753_27625)	70803..71033	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CW753_RS27450 (CW753_27640)	71629..71847	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	-
CW753_RS27455 (CW753_27645)	71849..72154	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	-
CW753_RS27460 (CW753_27650)	72312..72818	+	507	WP_023294776.1	hypothetical protein	-
CW753_RS27465 (CW753_27655)	72997..73773	+	777	WP_049245386.1	site-specific integrase	-
CW753_RS27470 (CW753_27660)	73831..74088	-	258	WP_000764642.1	hypothetical protein	-
CW753_RS29680	74217..74321	-	105	WP_032409716.1	hypothetical protein	-
CW753_RS27475 (CW753_27665)	74855..75721	+	867	WP_004118283.1	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..103957	103957

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15094.58 Da
• Isoelectric Point: 8.5403

>T89892 WP_048295728.1 NZ_CP025213:c70806-70390 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPALVLEDWVK

Nucleotide

• Length: 417 bp

>T89892 NZ_CP025213:c70806-70390 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCTGCCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT89892 WP_001261276.1 NZ_CP025213:c71033-70803 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT89892 NZ_CP025213:c71033-70803 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023