Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 38153..38889 Replicon plasmid unnamed1
Accession NZ_CP025212
Organism Klebsiella pneumoniae strain HZW25

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CW753_RS26455 Protein ID WP_003026803.1
Coordinates 38153..38635 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CW753_RS26460 Protein ID WP_003026799.1
Coordinates 38623..38889 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CW753_RS26440 (CW753_26610) 34562..35350 + 789 WP_134906156.1 transporter substrate-binding domain-containing protein -
CW753_RS26445 (CW753_26615) 35372..36340 + 969 WP_167553402.1 IS5 family transposase -
CW753_RS26450 (CW753_26620) 36462..37994 + 1533 WP_015065592.1 IS3-like element ISKpn38 family transposase -
CW753_RS26455 (CW753_26625) 38153..38635 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CW753_RS26460 (CW753_26630) 38623..38889 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CW753_RS26470 (CW753_26640) 39334..39564 - 231 WP_004206660.1 hypothetical protein -
CW753_RS26475 (CW753_26645) 39578..39781 - 204 WP_004213596.1 HHA domain-containing protein -
CW753_RS26480 (CW753_26650) 39842..40333 - 492 WP_015065591.1 hypothetical protein -
CW753_RS26485 (CW753_26655) 40467..41501 - 1035 WP_015065590.1 hypothetical protein -
CW753_RS26490 (CW753_26660) 41803..42807 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..145759 145759
- inside IScluster/Tn - - 35372..45371 9999


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T89891 WP_003026803.1 NZ_CP025212:c38635-38153 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T89891 NZ_CP025212:c38635-38153 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT89891 WP_003026799.1 NZ_CP025212:c38889-38623 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT89891 NZ_CP025212:c38889-38623 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References