Detailed information of TA system TA089889

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5070172..5070941
• Replicon: chromosome
• Accession: NZ_CP025211
• Organism: Klebsiella pneumoniae strain HZW25

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CW753_RS25580
• Protein ID: WP_134906132.1
• Coordinates: 5070555..5070941 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: CW753_RS25575
• Protein ID: WP_209300255.1
• Coordinates: 5070172..5070498 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CW753_RS25540 (CW753_25710)	5065355..5066002	-	648	WP_134906126.1	inovirus Gp2 family protein	-
CW753_RS25545 (CW753_25715)	5066571..5067208	+	638	Protein_4882	IS1 family transposase	-
CW753_RS25550 (CW753_25720)	5067236..5067709	-	474	WP_134906127.1	hypothetical protein	-
CW753_RS25555 (CW753_25725)	5068085..5068906	+	822	WP_134906128.1	DUF932 domain-containing protein	-
CW753_RS25560 (CW753_25730)	5068939..5069388	+	450	WP_134906129.1	antirestriction protein	-
CW753_RS25565 (CW753_25735)	5069400..5069879	+	480	WP_080925053.1	DNA repair protein RadC	-
CW753_RS25570 (CW753_25740)	5069893..5070114	+	222	WP_134906130.1	DUF987 domain-containing protein	-
CW753_RS25575 (CW753_25745)	5070172..5070498	+	327	WP_209300255.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CW753_RS25580 (CW753_25750)	5070555..5070941	+	387	WP_134906132.1	TA system toxin CbtA family protein	Toxin
CW753_RS25585 (CW753_25755)	5071028..5071879	+	852	WP_134906133.1	DUF4942 domain-containing protein	-
CW753_RS25590 (CW753_25760)	5072285..5073673	-	1389	WP_020804155.1	MFS transporter	-
CW753_RS25595 (CW753_25765)	5073803..5074732	+	930	WP_134906134.1	LysR family transcriptional regulator	-
CW753_RS25600 (CW753_25770)	5075096..5075899	-	804	WP_014906953.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5066571..5066843	272

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (8-110)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14398.47 Da
• Isoelectric Point: 8.0784

>T89889 WP_134906132.1 NZ_CP025211:5070555-5070941 [Klebsiella pneumoniae]
MQTKLPFMRAASSRPSPVDVWQTLLTCLLEHHYGLTLNDTPFSDELVIQQHIDAGISLADALNFIVEKYELVRTDRPGFS
IREQSPFITAIDILWARKATGLMNRGTYKEVTAITRGQHPQASTSGKR

Nucleotide

• Length: 387 bp

>T89889 NZ_CP025211:5070555-5070941 [Klebsiella pneumoniae]
ATGCAAACGAAATTACCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTTGATGTCTGGCAGACTCTCCTGACCTG
CCTGCTGGAACACCACTATGGTCTCACGCTTAACGATACGCCGTTCAGTGATGAACTGGTGATTCAGCAACATATCGACG
CGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTTGAGAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTCAGC
ATCAGGGAGCAGTCTCCGTTTATTACGGCCATTGATATTCTCTGGGCCAGAAAGGCTACAGGGCTGATGAACCGTGGCAC
GTATAAAGAGGTGACGGCTATTACCAGAGGACAACATCCGCAGGCCAGCACTTCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12104.75 Da
• Isoelectric Point: 6.6283

>AT89889 WP_209300255.1 NZ_CP025211:5070172-5070498 [Klebsiella pneumoniae]
ISDPWWGLRRSVSPCFGARLVQEGNRLHYLADRANFSGQFCDADLRHLDQAFPVLMKQLELMLTSGELNPRHQHGVTLYA
KGLTCEADTLGSHGYVYIAIYPAPVTTE

Nucleotide

• Length: 327 bp

>AT89889 NZ_CP025211:5070172-5070498 [Klebsiella pneumoniae]
ATCAGCGACCCCTGGTGGGGACTCAGGCGCAGTGTTTCCCCGTGTTTTGGGGCCCGTCTGGTCCAGGAGGGAAACCGGCT
GCATTACCTGGCGGATCGGGCGAATTTTAGCGGGCAGTTCTGTGATGCTGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TATTGATGAAACAGCTGGAGTTAATGCTTACCAGCGGTGAGCTGAATCCCCGCCACCAGCATGGCGTCACGCTGTATGCA
AAAGGCCTGACCTGCGAGGCCGATACGCTCGGTTCGCATGGCTACGTTTATATCGCGATTTATCCCGCTCCCGTCACCAC
AGAATAA