Detailed information of TA system TA089888

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 5038548..5039205
• Replicon: chromosome
• Accession: NZ_CP025211
• Organism: Klebsiella pneumoniae strain HZW25

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: CW753_RS25415
• Protein ID: WP_134906113.1
• Coordinates: 5038795..5039205 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: CW753_RS25410
• Protein ID: WP_002916312.1
• Coordinates: 5038548..5038814 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CW753_RS25385 (CW753_25555)	5033704..5035137	-	1434	WP_004185559.1	6-phospho-beta-glucosidase BglA	-
CW753_RS25390 (CW753_25560)	5035256..5035984	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
CW753_RS25395 (CW753_25565)	5036034..5036345	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
CW753_RS25400 (CW753_25570)	5036509..5037168	+	660	WP_002916317.1	hemolysin III family protein	-
CW753_RS25405 (CW753_25575)	5037319..5038302	-	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
CW753_RS25410 (CW753_25580)	5038548..5038814	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
CW753_RS25415 (CW753_25585)	5038795..5039205	+	411	WP_134906113.1	protein YgfX	Toxin
CW753_RS25420 (CW753_25590)	5039212..5039733	-	522	WP_004144730.1	flavodoxin FldB	-
CW753_RS25425 (CW753_25595)	5039834..5040730	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
CW753_RS25430 (CW753_25600)	5040753..5041466	+	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
CW753_RS25435 (CW753_25605)	5041472..5043205	+	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16035.83 Da
• Isoelectric Point: 11.4778

>T89888 WP_134906113.1 NZ_CP025211:5038795-5039205 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGDWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T89888 NZ_CP025211:5038795-5039205 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGATTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT89888 WP_002916312.1 NZ_CP025211:5038548-5038814 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT89888 NZ_CP025211:5038548-5038814 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41