Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2960257..2960876 Replicon chromosome
Accession NZ_CP025211
Organism Klebsiella pneumoniae strain HZW25

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CW753_RS15020 Protein ID WP_002892050.1
Coordinates 2960658..2960876 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CW753_RS15015 Protein ID WP_002892066.1
Coordinates 2960257..2960631 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CW753_RS15005 (CW753_15015) 2955409..2956602 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CW753_RS15010 (CW753_15020) 2956625..2959771 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CW753_RS15015 (CW753_15025) 2960257..2960631 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CW753_RS15020 (CW753_15030) 2960658..2960876 + 219 WP_002892050.1 HHA domain-containing protein Toxin
CW753_RS15025 (CW753_15035) 2961035..2961601 + 567 WP_002892030.1 maltose O-acetyltransferase -
CW753_RS29255 2961573..2961713 - 141 WP_004147370.1 hypothetical protein -
CW753_RS15030 (CW753_15040) 2961734..2962204 + 471 WP_002892026.1 YlaC family protein -
CW753_RS15035 (CW753_15045) 2962179..2963630 - 1452 WP_021313732.1 PLP-dependent aminotransferase family protein -
CW753_RS15040 (CW753_15050) 2963731..2964429 + 699 WP_002892021.1 GNAT family protein -
CW753_RS15045 (CW753_15055) 2964426..2964566 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CW753_RS15050 (CW753_15060) 2964566..2964829 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T89881 WP_002892050.1 NZ_CP025211:2960658-2960876 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T89881 NZ_CP025211:2960658-2960876 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT89881 WP_002892066.1 NZ_CP025211:2960257-2960631 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT89881 NZ_CP025211:2960257-2960631 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References