Detailed information of TA system TA089879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2120652..2121283
• Replicon: chromosome
• Accession: NZ_CP025211
• Organism: Klebsiella pneumoniae strain HZW25

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1Q8YTV3
• Locus tag: CW753_RS10805
• Protein ID: WP_012542177.1
• Coordinates: 2121107..2121283 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CW753_RS10800
• Protein ID: WP_101856539.1
• Coordinates: 2120652..2121059 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CW753_RS10755 (CW753_10730)	2116013..2116444	-	432	WP_077259664.1	hypothetical protein	-
CW753_RS10760 (CW753_10735)	2116441..2116758	-	318	WP_014228902.1	hypothetical protein	-
CW753_RS10765 (CW753_10740)	2116710..2117072	-	363	WP_017898990.1	HNH endonuclease signature motif containing protein	-
CW753_RS10770 (CW753_10745)	2117226..2117450	-	225	WP_223177722.1	DNA gyrase subunit B	-
CW753_RS29955 (CW753_10750)	2117555..2117743	-	189	WP_071888095.1	hypothetical protein	-
CW753_RS10785 (CW753_10760)	2118824..2119174	-	351	WP_017898986.1	hypothetical protein	-
CW753_RS10790 (CW753_10765)	2119171..2119668	-	498	WP_032439908.1	lysozyme	-
CW753_RS10795 (CW753_10770)	2119646..2119915	-	270	WP_032439907.1	phage holin	-
CW753_RS10800 (CW753_10775)	2120652..2121059	-	408	WP_101856539.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CW753_RS10805 (CW753_10780)	2121107..2121283	-	177	WP_012542177.1	type II toxin-antitoxin system HicA family toxin	Toxin
CW753_RS29080 (CW753_10785)	2121648..2121902	+	255	WP_167553390.1	hypothetical protein	-
CW753_RS10815 (CW753_10795)	2122002..2122982	-	981	WP_000019449.1	IS5-like element ISKpn26 family transposase	-
CW753_RS10820 (CW753_10800)	2123036..2123992	+	957	WP_167553391.1	nucleoid-associated protein	-
CW753_RS10825 (CW753_10805)	2123982..2125208	+	1227	WP_064147745.1	hypothetical protein	-
CW753_RS10830 (CW753_10810)	2125203..2125547	-	345	WP_064147746.1	antiterminator Q family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2087186..2145111	57925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6632.83 Da
• Isoelectric Point: 11.0174

>T89879 WP_012542177.1 NZ_CP025211:c2121283-2121107 [Klebsiella pneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Nucleotide

• Length: 177 bp

>T89879 NZ_CP025211:c2121283-2121107 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGCATCAAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14992.02 Da
• Isoelectric Point: 4.4277

>AT89879 WP_101856539.1 NZ_CP025211:c2121059-2120652 [Klebsiella pneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIIVH

Nucleotide

• Length: 408 bp

>AT89879 NZ_CP025211:c2121059-2120652 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAAGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCATGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGCACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTATCG
TCCACTAA

Structures

Toxin

• AlphaFold DB: A0A1Q8YTV3