Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 114255..114991 Replicon plasmid NR5632_p2
Accession NZ_CP025145
Organism Klebsiella pneumoniae strain NR5632

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CXB25_RS29145 Protein ID WP_003026803.1
Coordinates 114255..114737 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CXB25_RS29150 Protein ID WP_003026799.1
Coordinates 114725..114991 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXB25_RS29130 109892..110854 + 963 WP_004152113.1 M48 family metalloprotease -
CXB25_RS29135 110942..112196 + 1255 Protein_123 IS3 family transposase -
CXB25_RS29140 112697..114043 - 1347 WP_020314316.1 ISNCY family transposase -
CXB25_RS29145 114255..114737 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CXB25_RS29150 114725..114991 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CXB25_RS29155 115167..115421 - 255 WP_004152108.1 hypothetical protein -
CXB25_RS29160 115497..115754 - 258 WP_004152107.1 hypothetical protein -
CXB25_RS29165 115803..116006 - 204 WP_004152106.1 hemolysin expression modulator Hha -
CXB25_RS29170 116040..116408 - 369 WP_004152105.1 hypothetical protein -
CXB25_RS29175 116452..116946 - 495 WP_004152104.1 DNA-binding protein -
CXB25_RS29180 116977..117552 - 576 WP_004152103.1 hypothetical protein -
CXB25_RS29185 117540..117809 - 270 WP_004152102.1 hypothetical protein -
CXB25_RS29190 118167..118517 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CXB25_RS29195 118567..118929 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..149158 149158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T89832 WP_003026803.1 NZ_CP025145:c114737-114255 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T89832 NZ_CP025145:c114737-114255 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT89832 WP_003026799.1 NZ_CP025145:c114991-114725 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT89832 NZ_CP025145:c114991-114725 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References