Detailed information of TA system TA089828

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4935954..4936657
• Replicon: chromosome
• Accession: NZ_CP025143
• Organism: Klebsiella pneumoniae strain NR5632

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A939NMR5
• Locus tag: CXB25_RS24830
• Protein ID: WP_071994632.1
• Coordinates: 4936316..4936657 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A939NIK9
• Locus tag: CXB25_RS24825
• Protein ID: WP_032434296.1
• Coordinates: 4935954..4936295 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXB25_RS24790	4931109..4931983	+	875	Protein_4720	GTPase family protein	-
CXB25_RS24795	4932227..4932943	+	717	WP_032434305.1	WYL domain-containing protein	-
CXB25_RS24800	4932979..4933431	+	453	WP_032410767.1	hypothetical protein	-
CXB25_RS24805	4933503..4933976	+	474	WP_032434303.1	hypothetical protein	-
CXB25_RS24810	4934096..4934917	+	822	WP_032434301.1	DUF945 domain-containing protein	-
CXB25_RS24815	4934948..4935388	+	441	WP_032434300.1	antirestriction protein	-
CXB25_RS24820	4935401..4935943	+	543	WP_032434298.1	DNA repair protein RadC	-
CXB25_RS24825	4935954..4936295	+	342	WP_032434296.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CXB25_RS24830	4936316..4936657	+	342	WP_071994632.1	TA system toxin CbtA family protein	Toxin
CXB25_RS24835	4936849..4938882	-	2034	WP_050598589.1	hypothetical protein	-
CXB25_RS24840	4939473..4940342	-	870	WP_023317468.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4903360..4950381	47021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12789.77 Da
• Isoelectric Point: 9.6552

>T89828 WP_071994632.1 NZ_CP025143:4936316-4936657 [Klebsiella pneumoniae]
MKTLPATTPQAVTLCLSSVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRKGF
NWQEHSPYLRAVDILRARQATGLLRRSRKNAVR

Nucleotide

• Length: 342 bp

>T89828 NZ_CP025143:4936316-4936657 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12893.40 Da
• Isoelectric Point: 5.9612

>AT89828 WP_032434296.1 NZ_CP025143:4935954-4936295 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGEFSNAESLKLDEVFPHFISQMESMLTTGEMNPRH
AHFFTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT89828 NZ_CP025143:4935954-4936295 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA