Detailed information of TA system TA089827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4843413..4843929
• Replicon: chromosome
• Accession: NZ_CP025143
• Organism: Klebsiella pneumoniae strain NR5632

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4YAY3
• Locus tag: CXB25_RS24415
• Protein ID: WP_004178374.1
• Coordinates: 4843645..4843929 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A919M0P8
• Locus tag: CXB25_RS24410
• Protein ID: WP_032434351.1
• Coordinates: 4843413..4843655 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXB25_RS24395	4839442..4840182	+	741	WP_002886899.1	KDGP aldolase family protein	-
CXB25_RS24400	4840248..4841402	+	1155	WP_002886900.1	lactonase family protein	-
CXB25_RS24405	4841425..4843335	+	1911	WP_101134870.1	BglG family transcription antiterminator	-
CXB25_RS24410	4843413..4843655	+	243	WP_032434351.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
CXB25_RS24415	4843645..4843929	+	285	WP_004178374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXB25_RS24420	4843933..4844397	-	465	WP_004222151.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
CXB25_RS24425	4844639..4846777	-	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
CXB25_RS24430	4847134..4847877	-	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
CXB25_RS30065	4847880..4848053	-	174	WP_032414379.1	hypothetical protein	-
CXB25_RS24435	4848138..4848446	+	309	WP_012737232.1	PTS sugar transporter subunit IIB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-68)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11155.97 Da
• Isoelectric Point: 10.3787

>T89827 WP_004178374.1 NZ_CP025143:4843645-4843929 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T89827 NZ_CP025143:4843645-4843929 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9003.47 Da
• Isoelectric Point: 6.3340

>AT89827 WP_032434351.1 NZ_CP025143:4843413-4843655 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGGKVSLDDL

Nucleotide

• Length: 243 bp

>AT89827 NZ_CP025143:4843413-4843655 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A6THG1