Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 117327..118063 Replicon plasmid KP1768_p2
Accession NZ_CP025142
Organism Klebsiella pneumoniae strain KP1768

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CXB24_RS29145 Protein ID WP_003026803.1
Coordinates 117327..117809 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CXB24_RS29150 Protein ID WP_003026799.1
Coordinates 117797..118063 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXB24_RS29130 112964..113926 + 963 WP_004152113.1 M48 family metalloprotease -
CXB24_RS29135 114014..115268 + 1255 Protein_126 IS3 family transposase -
CXB24_RS29140 115769..117115 - 1347 WP_020314316.1 ISNCY family transposase -
CXB24_RS29145 117327..117809 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CXB24_RS29150 117797..118063 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CXB24_RS29155 118239..118493 - 255 WP_004152108.1 hypothetical protein -
CXB24_RS29160 118569..118826 - 258 WP_004152107.1 hypothetical protein -
CXB24_RS29165 118875..119078 - 204 WP_004152106.1 hemolysin expression modulator Hha -
CXB24_RS29170 119112..119480 - 369 WP_004152105.1 hypothetical protein -
CXB24_RS29175 119524..120018 - 495 WP_004152104.1 DNA-binding protein -
CXB24_RS29180 120049..120624 - 576 WP_004152103.1 hypothetical protein -
CXB24_RS29185 120612..120881 - 270 WP_004152102.1 hypothetical protein -
CXB24_RS29190 121239..121589 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CXB24_RS29195 121639..122001 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..152230 152230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T89811 WP_003026803.1 NZ_CP025142:c117809-117327 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T89811 NZ_CP025142:c117809-117327 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT89811 WP_003026799.1 NZ_CP025142:c118063-117797 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT89811 NZ_CP025142:c118063-117797 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References