Detailed information of TA system TA089807

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4935802..4936505
• Replicon: chromosome
• Accession: NZ_CP025140
• Organism: Klebsiella pneumoniae strain KP1768

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A939NMR5
• Locus tag: CXB24_RS24810
• Protein ID: WP_071994632.1
• Coordinates: 4936164..4936505 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A939NIK9
• Locus tag: CXB24_RS24805
• Protein ID: WP_032434296.1
• Coordinates: 4935802..4936143 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXB24_RS24770	4930957..4931831	+	875	Protein_4718	GTPase family protein	-
CXB24_RS24775	4932075..4932791	+	717	WP_032434305.1	WYL domain-containing protein	-
CXB24_RS24780	4932827..4933279	+	453	WP_032410767.1	hypothetical protein	-
CXB24_RS24785	4933351..4933824	+	474	WP_032434303.1	hypothetical protein	-
CXB24_RS24790	4933944..4934765	+	822	WP_032434301.1	DUF945 domain-containing protein	-
CXB24_RS24795	4934796..4935236	+	441	WP_032434300.1	antirestriction protein	-
CXB24_RS24800	4935249..4935791	+	543	WP_032434298.1	DNA repair protein RadC	-
CXB24_RS24805	4935802..4936143	+	342	WP_032434296.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CXB24_RS24810	4936164..4936505	+	342	WP_071994632.1	TA system toxin CbtA family protein	Toxin
CXB24_RS24815	4936697..4938730	-	2034	WP_050598589.1	hypothetical protein	-
CXB24_RS24820	4939321..4940190	-	870	WP_023317468.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4902535..4950217	47682

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12789.77 Da
• Isoelectric Point: 9.6552

>T89807 WP_071994632.1 NZ_CP025140:4936164-4936505 [Klebsiella pneumoniae]
MKTLPATTPQAVTLCLSSVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRKGF
NWQEHSPYLRAVDILRARQATGLLRRSRKNAVR

Nucleotide

• Length: 342 bp

>T89807 NZ_CP025140:4936164-4936505 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12893.40 Da
• Isoelectric Point: 5.9612

>AT89807 WP_032434296.1 NZ_CP025140:4935802-4936143 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGEFSNAESLKLDEVFPHFISQMESMLTTGEMNPRH
AHFFTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT89807 NZ_CP025140:4935802-4936143 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA