Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 5038298..5039273 Replicon chromosome
Accession NZ_CP025122
Organism Bacillus sp. HBCD-sjtu

Toxin (Protein)


Gene name spoIISA Uniprot ID C3GJ28
Locus tag CU648_RS27230 Protein ID WP_003297560.1
Coordinates 5038298..5039035 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag CU648_RS27235 Protein ID WP_000588712.1
Coordinates 5039148..5039273 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU648_RS27200 5033373..5034083 + 711 WP_000787479.1 class I SAM-dependent methyltransferase -
CU648_RS27205 5034203..5034610 + 408 WP_000072487.1 VOC family protein -
CU648_RS27210 5034930..5036615 - 1686 WP_031302169.1 alpha-keto acid decarboxylase family protein -
CU648_RS27215 5036722..5037204 + 483 WP_000191911.1 MarR family winged helix-turn-helix transcriptional regulator -
CU648_RS27220 5037372..5038109 + 738 WP_000594156.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
CU648_RS27230 5038298..5039035 + 738 WP_003297560.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
CU648_RS27235 5039148..5039273 + 126 WP_000588712.1 hypothetical protein Antitoxin
CU648_RS27240 5039349..5039525 + 177 WP_000852620.1 stage II sporulation protein SB -
CU648_RS27245 5039544..5039933 - 390 WP_000808873.1 YxeA family protein -
CU648_RS27250 5040149..5041618 + 1470 WP_017560293.1 beta-Ala-His dipeptidase -
CU648_RS27255 5041853..5042323 - 471 WP_000670597.1 DUF5065 family protein -
CU648_RS27260 5042500..5043120 + 621 WP_147792116.1 SGNH/GDSL hydrolase family protein -
CU648_RS27265 5043342..5043875 + 534 WP_000438332.1 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28373.07 Da        Isoelectric Point: 8.2672

>T89789 WP_003297560.1 NZ_CP025122:5038298-5039035 [Bacillus sp. HBCD-sjtu]
VISNIRIGLFILAIVFLILVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T89789 NZ_CP025122:5038298-5039035 [Bacillus sp. HBCD-sjtu]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAATACTTGTTTTCTTTTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTAGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT89789 WP_000588712.1 NZ_CP025122:5039148-5039273 [Bacillus sp. HBCD-sjtu]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT89789 NZ_CP025122:5039148-5039273 [Bacillus sp. HBCD-sjtu]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3R9EK80

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References