Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2888094..2888736 Replicon chromosome
Accession NZ_CP025122
Organism Bacillus sp. HBCD-sjtu

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag CU648_RS15805 Protein ID WP_000635963.1
Coordinates 2888386..2888736 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag CU648_RS15800 Protein ID WP_000004570.1
Coordinates 2888094..2888381 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU648_RS15775 2883410..2884372 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
CU648_RS15780 2884365..2884937 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
CU648_RS15785 2885030..2885389 + 360 WP_000635040.1 holo-ACP synthase -
CU648_RS15790 2885546..2886496 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
CU648_RS15795 2886615..2887784 + 1170 WP_000390600.1 alanine racemase -
CU648_RS15800 2888094..2888381 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
CU648_RS15805 2888386..2888736 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CU648_RS15810 2888804..2890972 + 2169 WP_139371941.1 RNA-binding transcriptional accessory protein -
CU648_RS15815 2891030..2891146 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
CU648_RS15820 2891342..2891800 + 459 WP_000344246.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T89788 WP_000635963.1 NZ_CP025122:2888386-2888736 [Bacillus sp. HBCD-sjtu]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T89788 NZ_CP025122:2888386-2888736 [Bacillus sp. HBCD-sjtu]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT89788 WP_000004570.1 NZ_CP025122:2888094-2888381 [Bacillus sp. HBCD-sjtu]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT89788 NZ_CP025122:2888094-2888381 [Bacillus sp. HBCD-sjtu]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References