Detailed information of TA system TA089753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 155987..156657
• Replicon: plasmid pSGH10
• Accession: NZ_CP025081
• Organism: Klebsiella pneumoniae strain SGH10

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: SGH10_RS28025
• Protein ID: WP_004213072.1
• Coordinates: 155987..156430 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: SGH10_RS28030
• Protein ID: WP_004213073.1
• Coordinates: 156427..156657 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SGH10_RS27975	151394..151669	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
SGH10_RS27980	151732..152223	+	492	WP_011251327.1	DM13 domain-containing protein	-
SGH10_RS27985	152272..153192	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
SGH10_RS27995	153283..153689	+	407	Protein_162	GAF domain-containing protein	-
SGH10_RS29255	154204..154842	-	639	Protein_163	response regulator	-
SGH10_RS28015	155260..155553	+	294	Protein_164	transposase	-
SGH10_RS28020	155557..155973	+	417	Protein_165	restriction endonuclease	-
SGH10_RS28025	155987..156430	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
SGH10_RS28030	156427..156657	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SGH10_RS28035	157265..158398	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
SGH10_RS28040	158414..158707	+	294	WP_004213076.1	hypothetical protein	-
SGH10_RS28045	158697..158903	-	207	WP_004213077.1	hypothetical protein	-
SGH10_RS28050	159255..159545	+	291	WP_004213078.1	hypothetical protein	-
SGH10_RS28055	159535..160434	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..231583	231583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T89753 WP_004213072.1 NZ_CP025081:c156430-155987 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T89753 NZ_CP025081:c156430-155987 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT89753 WP_004213073.1 NZ_CP025081:c156657-156427 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT89753 NZ_CP025081:c156657-156427 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620