Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 515374..516011 Replicon chromosome
Accession NZ_CP025079
Organism Bacillus velezensis strain QST713

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BVQ_RS02460 Protein ID WP_003156187.1
Coordinates 515661..516011 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BVQ_RS02455 Protein ID WP_003156188.1
Coordinates 515374..515655 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BVQ_RS02435 511739..512338 - 600 WP_053573675.1 rhomboid family intramembrane serine protease -
BVQ_RS02440 512431..512796 + 366 WP_015416872.1 holo-ACP synthase -
BVQ_RS02445 512961..513968 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
BVQ_RS02450 514085..515254 + 1170 WP_053573676.1 alanine racemase -
BVQ_RS02455 515374..515655 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BVQ_RS02460 515661..516011 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BVQ_RS02465 516129..516950 + 822 WP_033575045.1 STAS domain-containing protein -
BVQ_RS02470 516955..517320 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
BVQ_RS02475 517323..517724 + 402 WP_003156178.1 anti-sigma regulatory factor -
BVQ_RS02480 517736..518743 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
BVQ_RS02485 518807..519136 + 330 WP_033575044.1 anti-sigma factor antagonist -
BVQ_RS02490 519133..519615 + 483 WP_007609591.1 anti-sigma B factor RsbW -
BVQ_RS02495 519581..520369 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
BVQ_RS02500 520369..520971 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T89733 WP_003156187.1 NZ_CP025079:515661-516011 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T89733 NZ_CP025079:515661-516011 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT89733 WP_003156188.1 NZ_CP025079:515374-515655 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT89733 NZ_CP025079:515374-515655 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References