Detailed information of TA system TA089731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 12850..13987
• Replicon: plasmid unnamed
• Accession: NZ_CP025078
• Organism: Enterococcus faecium strain LS170308

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: CWE29_RS13520
• Protein ID: WP_024417198.1
• Coordinates: 13124..13987 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: CWE29_RS13515
• Protein ID: WP_000301765.1
• Coordinates: 12850..13122 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWE29_RS13495	8558..9877	+	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-
CWE29_RS13500	10068..11555	+	1488	Protein_11	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
CWE29_RS13505	11658..12554	+	897	WP_010725963.1	ParA family protein	-
CWE29_RS13510	12624..12833	+	210	WP_000527318.1	peptide-binding protein	-
CWE29_RS13515	12850..13122	+	273	WP_000301765.1	antitoxin	Antitoxin
CWE29_RS13520	13124..13987	+	864	WP_024417198.1	zeta toxin family protein	Toxin
CWE29_RS13525	14109..14606	+	498	WP_096710545.1	molecular chaperone DnaJ	-
CWE29_RS13530	14691..15287	-	597	WP_002326773.1	TetR/AcrR family transcriptional regulator	-
CWE29_RS13535	15598..16479	+	882	WP_098379806.1	ABC transporter ATP-binding protein	-
CWE29_RS13540	16503..18116	+	1614	WP_098379807.1	tetronasin resistance protein	-
CWE29_RS13545	18188..18700	-	513	Protein_20	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	lsa(E) / lnu(B) / ant(6)-Ia / erm(B) / tet(M) / tet(L) / cat	-	1..56373	56373
	-	inside	IS/Tn	lsa(E) / lnu(B) / ant(6)-Ia	-	1047..9877	8830

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32404.02 Da
• Isoelectric Point: 6.9964

>T89731 WP_024417198.1 NZ_CP025078:13124-13987 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T89731 NZ_CP025078:13124-13987 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGACTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT89731 WP_000301765.1 NZ_CP025078:12850-13122 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT89731 NZ_CP025078:12850-13122 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3