Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-yefM/Txe-RelB
Location 2435018..2435547 Replicon chromosome
Accession NZ_CP025077
Organism Enterococcus faecium strain LS170308

Toxin (Protein)


Gene name relE Uniprot ID C9B8W9
Locus tag CWE29_RS12410 Protein ID WP_002294198.1
Coordinates 2435018..2435284 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C9B8W8
Locus tag CWE29_RS12415 Protein ID WP_002294199.1
Coordinates 2435284..2435547 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWE29_RS12385 2430385..2431560 + 1176 WP_002294192.1 betaine/proline/choline family ABC transporter ATP-binding protein -
CWE29_RS12390 2431564..2432205 + 642 WP_002293405.1 ABC transporter permease -
CWE29_RS12395 2432202..2433119 + 918 WP_002298332.1 osmoprotectant ABC transporter substrate-binding protein -
CWE29_RS12400 2433116..2433787 + 672 WP_002319469.1 ABC transporter permease -
CWE29_RS12405 2434329..2434817 - 489 WP_002296561.1 mannosyl-glycoprotein endo-beta-N-acetylglucosamidase -
CWE29_RS12410 2435018..2435284 - 267 WP_002294198.1 Txe/YoeB family addiction module toxin Toxin
CWE29_RS12415 2435284..2435547 - 264 WP_002294199.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CWE29_RS12430 2436178..2437368 - 1191 WP_002298336.1 arabinan endo-1,5-alpha-L-arabinosidase -
CWE29_RS12435 2437421..2437687 - 267 WP_002315578.1 hypothetical protein -
CWE29_RS12440 2437692..2439137 - 1446 WP_002337067.1 alpha-N-arabinofuranosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(22-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10646.11 Da        Isoelectric Point: 9.9931

>T89729 WP_002294198.1 NZ_CP025077:c2435284-2435018 [Enterococcus faecium]
MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQSFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEI
YSAWTHYE

Download         Length: 267 bp

>T89729 NZ_CP025077:c2435284-2435018 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10016.03 Da        Isoelectric Point: 4.1207

>AT89729 WP_002294199.1 NZ_CP025077:c2435547-2435284 [Enterococcus faecium]
MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIGLEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNID
DIDWEAL

Download         Length: 264 bp

>AT89729 NZ_CP025077:c2435547-2435284 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGATGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FMA0

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZU84

References