Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2118632..2119203 Replicon chromosome
Accession NZ_CP025077
Organism Enterococcus faecium strain LS170308

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag CWE29_RS10845 Protein ID WP_002302307.1
Coordinates 2118632..2118973 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag CWE29_RS10850 Protein ID WP_002323011.1
Coordinates 2118973..2119203 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWE29_RS10835 2113663..2116166 - 2504 Protein_1957 hypothetical protein -
CWE29_RS10840 2116497..2117659 + 1163 WP_098188817.1 IS3 family transposase -
CWE29_RS10845 2118632..2118973 - 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CWE29_RS10850 2118973..2119203 - 231 WP_002323011.1 hypothetical protein Antitoxin
CWE29_RS10860 2119491..2119829 + 339 WP_100970561.1 hypothetical protein -
CWE29_RS10865 2120034..2120609 - 576 WP_002302305.1 SOS response-associated peptidase family protein -
CWE29_RS10880 2120973..2121554 - 582 WP_002300332.1 TetR/AcrR family transcriptional regulator -
CWE29_RS10885 2121747..2122373 - 627 WP_002300333.1 cysteine hydrolase -
CWE29_RS10890 2122395..2123726 - 1332 WP_002300334.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T89728 WP_002302307.1 NZ_CP025077:c2118973-2118632 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T89728 NZ_CP025077:c2118973-2118632 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT89728 WP_002323011.1 NZ_CP025077:c2119203-2118973 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT89728 NZ_CP025077:c2119203-2118973 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References