Detailed information of TA system TA089726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 810073..810741
• Replicon: chromosome
• Accession: NZ_CP025076
• Organism: Streptococcus pneumoniae strain BHN97x

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: I0T4C6
• Locus tag: CWI64_RS04255
• Protein ID: WP_001132287.1
• Coordinates: 810562..810741 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G0IC64
• Locus tag: CWI64_RS04250
• Protein ID: WP_000961810.1
• Coordinates: 810073..810525 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI64_RS04230	806922..807956	-	1035	WP_000747915.1	ATP-binding protein	-
CWI64_RS04235	808066..808224	-	159	WP_000401986.1	7,8-dihydro-8-oxoguanine-triphosphatase	-
CWI64_RS04240	808226..809293	-	1068	WP_000719703.1	M50 family metallopeptidase	-
CWI64_RS04245	809312..809782	-	471	WP_000257083.1	DUF3013 family protein	-
CWI64_RS04250	810073..810525	-	453	WP_000961810.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CWI64_RS04255	810562..810741	-	180	WP_001132287.1	type II toxin-antitoxin system HicA family toxin	Toxin
CWI64_RS04260	810878..811057	-	180	WP_001810816.1	hypothetical protein	-
CWI64_RS04270	811222..811461	-	240	WP_000818079.1	hypothetical protein	-
CWI64_RS04280	811731..813002	+	1272	WP_001113214.1	replication-associated recombination protein A	-
CWI64_RS04290	813549..814868	-	1320	WP_000502537.1	glycoside hydrolase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (17-114)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6697.91 Da
• Isoelectric Point: 11.0174

>T89726 WP_001132287.1 NZ_CP025076:c810741-810562 [Streptococcus pneumoniae]
MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITIPHGELNKYTERGIRKQAGL

Nucleotide

• Length: 180 bp

>T89726 NZ_CP025076:c810741-810562 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGTTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACCTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16659.46 Da
• Isoelectric Point: 3.7220

>AT89726 WP_000961810.1 NZ_CP025076:c810525-810073 [Streptococcus pneumoniae]
MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAMGSEWLGITVADLIESDGELPQPSDINSLSLIDNDPF
KDDEDFVSTYDLDKSFISMVSVDVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA

Nucleotide

• Length: 453 bp

>AT89726 NZ_CP025076:c810525-810073 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCCACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGATGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• AlphaFold DB: I0T4C6

Antitoxin

• AlphaFold DB: G0IC64