Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 1773154..1773790 Replicon chromosome
Accession NZ_CP025074
Organism [Bacillus] caldolyticus strain NEB414

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag CWI35_RS09210 Protein ID WP_003253417.1
Coordinates 1773154..1773504 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G8N2B5
Locus tag CWI35_RS09215 Protein ID WP_011229742.1
Coordinates 1773509..1773790 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWI35_RS09195 1770052..1770501 - 450 WP_119877683.1 SprT family protein -
CWI35_RS09200 1770591..1770704 + 114 WP_013144046.1 cortex morphogenetic protein CmpA -
CWI35_RS09205 1770723..1772888 - 2166 WP_119877684.1 RNA-binding transcriptional accessory protein -
CWI35_RS09210 1773154..1773504 - 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CWI35_RS09215 1773509..1773790 - 282 WP_011229742.1 hypothetical protein Antitoxin
CWI35_RS09220 1773917..1775071 - 1155 WP_011229741.1 alanine racemase -
CWI35_RS09225 1775320..1776333 - 1014 WP_014194683.1 outer membrane lipoprotein carrier protein LolA -
CWI35_RS09230 1776413..1776802 - 390 WP_011229738.1 holo-ACP synthase -
CWI35_RS09235 1776916..1777533 + 618 WP_014194682.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T89722 WP_003253417.1 NZ_CP025074:c1773504-1773154 [[Bacillus] caldolyticus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T89722 NZ_CP025074:c1773504-1773154 [[Bacillus] caldolyticus]
TTGATTGTCAAACGTGGCGACGTGTATTTTGCGGACCTTTCCCCGGTTGTTGGCTCGGAGCAGGGCGGCGTGCGCCCCGT
GTTGGTGATCCAAAACGATATCGGCAATCGTTTTAGCCCGACGGTGATTGTCGCGGCGATTACGGCGCAAATCCAAAAAG
CGAAGCTGCCGACGCATGTCGAGATTGACGCGAAACGCTACGGGTTTGAACGCGATTCGGTCATTTTGCTTGAGCAAATT
CGCACGATCGACAAGCAACGGCTGACCGATAAAATCACTCATTTGGACGATGAAATGATGGATAAAGTCGATGAGGCGCT
GCAAATTAGCTTAGGGCTGATCGACTTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10639.14 Da        Isoelectric Point: 7.2027

>AT89722 WP_011229742.1 NZ_CP025074:c1773790-1773509 [[Bacillus] caldolyticus]
VSESGATAEIVVRLPQSLLTELDVLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT89722 NZ_CP025074:c1773790-1773509 [[Bacillus] caldolyticus]
GTGTCGGAATCTGGCGCAACAGCGGAAATCGTCGTTCGTTTGCCGCAGTCGCTGCTGACAGAACTGGACGTGCTCGTAAA
GCAGGAAAACGGCAACCGCAATGAACTCATTTATCAAGCGACGAAAATGTACATTCGCGAGCGGAAGAAACGGCAAATTC
GCGAGGCGATGAGACGAGGCTACATGGAAATGGCGAAAATCAATTTATCTATCGCTTCTGAAGCGTTTCATGCTGAATAC
GAGGCCGACCACACCGTTGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9ETW5

References