89721

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 349988..350586 Replicon chromosome
Accession NZ_CP025074
Organism [Bacillus] caldolyticus strain NEB414

Toxin (Protein)


Gene name mazF Uniprot ID G8N5V4
Locus tag CWI35_RS01855 Protein ID WP_011231143.1
Coordinates 349988..350317 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G8N5V3
Locus tag CWI35_RS01860 Protein ID WP_011231142.1
Coordinates 350317..350586 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWI35_RS01830 345678..346856 - 1179 WP_013523725.1 acyl-CoA dehydrogenase family protein -
CWI35_RS01835 347037..347780 - 744 WP_119877433.1 NAD-dependent protein deacylase -
CWI35_RS01840 347854..348603 - 750 WP_068895474.1 oxygen-insensitive NADPH nitroreductase -
CWI35_RS01845 348699..349532 - 834 WP_068895472.1 pirin family protein -
CWI35_RS01855 349988..350317 - 330 WP_011231143.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CWI35_RS01860 350317..350586 - 270 WP_011231142.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CWI35_RS18390 351197..351403 + 207 Protein_346 hypothetical protein -
CWI35_RS18395 351470..351670 + 201 WP_198527193.1 hypothetical protein -
CWI35_RS01890 351801..353048 + 1248 WP_050368395.1 transposase -
CWI35_RS01895 353250..353603 + 354 WP_119877434.1 hypothetical protein -
CWI35_RS01900 353787..354767 - 981 WP_011231139.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-107)

Antitoxin

Antitoxin PemI-like

(12-87)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12071.86 Da        Isoelectric Point: 7.1331

>T89721 WP_011231143.1 NZ_CP025074:c350317-349988 [[Bacillus] caldolyticus]
MQAPDRGDLVYVNFNPQAGHEQAGKRPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVELPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHTFLS

Download         Length: 330 bp

>T89721 NZ_CP025074:c350317-349988 [[Bacillus] caldolyticus]
ATGCAAGCGCCGGATCGCGGGGATCTTGTTTACGTCAATTTCAATCCACAAGCAGGGCATGAGCAGGCAGGAAAAAGGCC
CGGCATCGTTCTATCACCAAAGCGGTTCAATCAATTGACAGGGTTTGCGGTGCTTTGCCCGATCACCCGGCAACAAAAAG
GATATCCATTTGAAGTGGAATTGCCATCAGGCTTGGCCGTTGAAGGCGTGATTTTAACCGATCAGGTCAAAAGTTTAGAT
TGGCGCGCCCGACAGCTTCAAATAGTAGGACGGGCGCCCAATGAAGTTGTTTCGGATTGCTTGGATCTGATTCATACTTT
TCTTTCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9992.67 Da        Isoelectric Point: 7.2050

>AT89721 WP_011231142.1 NZ_CP025074:c350586-350317 [[Bacillus] caldolyticus]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEMIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Download         Length: 270 bp

>AT89721 NZ_CP025074:c350586-350317 [[Bacillus] caldolyticus]
ATGGAATTAAACAAACAGAAAGGAGTGTCAATCATGACAATAACCGTTCAAAAATGGGGAAACAGCCTTGCGGTTCGTAT
CCCAAGCGTGATTGCTGAACGTTTAGCGCTTCATCAAGGATCAGAAGTGGAGATGATCGTTGAGAACCAAGCGATCAAGT
TGATCCCGAAAAAGAAAAAGCCGACATTGGAGGAACTTTTGGCTAAAATCACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9EUB7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9HWH2

References