Detailed information of TA system TA089718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3383330..3383966
• Replicon: chromosome
• Accession: NZ_CP025072
• Organism: Bordetella parapertussis strain B160

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q7W5Z1
• Locus tag: CWR60_RS15985
• Protein ID: WP_010928914.1
• Coordinates: 3383330..3383512 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q7W5Z0
• Locus tag: CWR60_RS15990
• Protein ID: WP_010928915.1
• Coordinates: 3383574..3383966 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR60_RS15960	3378904..3379581	+	678	WP_010928910.1	HAD-IA family hydrolase	-
CWR60_RS15965	3379799..3380986	+	1188	WP_010928911.1	MFS transporter	-
CWR60_RS15975	3381561..3382781	+	1221	WP_010928912.1	ISL3-like element IS1001 family transposase	-
CWR60_RS15985	3383330..3383512	+	183	WP_010928914.1	type II toxin-antitoxin system HicA family toxin	Toxin
CWR60_RS15990	3383574..3383966	+	393	WP_010928915.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CWR60_RS15995	3384239..3385148	-	910	Protein_3137	LysR family transcriptional regulator	-
CWR60_RS16000	3385273..3386247	+	975	WP_010928916.1	tripartite tricarboxylate transporter substrate binding protein	-
CWR60_RS16005	3386282..3386641	+	360	WP_010928917.1	hypothetical protein	-
CWR60_RS16010	3386708..3387880	+	1173	WP_010928918.1	thiolase domain-containing protein	-
CWR60_RS16015	3387884..3388310	+	427	Protein_3141	OB-fold domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3381561..3382781	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6572.69 Da
• Isoelectric Point: 10.9062

>T89718 WP_010928914.1 NZ_CP025072:3383330-3383512 [Bordetella parapertussis]
MDGKELIKQLERSGWTLRSVKGSHHVFAHPDRPGHISVPHPKKDLGIGLLNSILKAAGLK

Nucleotide

• Length: 183 bp

>T89718 NZ_CP025072:3383330-3383512 [Bordetella parapertussis]
ATGGACGGCAAAGAACTGATCAAGCAGCTTGAGCGGTCCGGGTGGACACTGCGGAGCGTTAAGGGCTCCCACCACGTTTT
CGCCCACCCAGACCGCCCCGGACATATCAGCGTCCCTCACCCTAAAAAGGATCTCGGCATCGGACTACTAAACAGCATCC
TCAAAGCAGCTGGTTTGAAGTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14058.82 Da
• Isoelectric Point: 4.2876

>AT89718 WP_010928915.1 NZ_CP025072:3383574-3383966 [Bordetella parapertussis]
VKYPIAIEPGSDTRAWGVVVPDLPGCFSAADEGIDQAIENAKEAIELWIETAIDSGAPIPAATNIANHQANPEFANWIWA
IADVDPAVLDETVERVNITIPRRILKRLDDRARAAGESRSSYIAHLAMTA

Nucleotide

• Length: 393 bp

>AT89718 NZ_CP025072:3383574-3383966 [Bordetella parapertussis]
GTGAAATATCCCATTGCCATTGAACCCGGCAGCGACACGCGCGCCTGGGGAGTCGTTGTTCCTGATCTGCCCGGCTGCTT
TTCCGCAGCGGACGAAGGGATTGATCAAGCAATCGAGAACGCCAAGGAAGCCATTGAGTTGTGGATTGAAACGGCCATCG
ACTCCGGCGCCCCCATTCCTGCGGCAACCAACATAGCCAATCATCAGGCCAATCCTGAGTTCGCCAACTGGATTTGGGCG
ATTGCTGATGTCGATCCTGCAGTCTTGGATGAAACTGTCGAGCGCGTGAACATCACAATCCCGCGCCGAATTCTCAAGCG
CCTGGATGATCGCGCCCGCGCCGCCGGCGAAAGCCGGAGCAGCTACATCGCACATCTCGCCATGACTGCCTGA

Structures

Toxin

• AlphaFold DB: Q7W5Z1

Antitoxin

• AlphaFold DB: Q7W5Z0