Detailed information of TA system TA089715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 2093108..2093639
• Replicon: chromosome
• Accession: NZ_CP025072
• Organism: Bordetella parapertussis strain B160

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: CWR60_RS09985
• Protein ID: WP_003812626.1
• Coordinates: 2093373..2093639 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: CWR60_RS09980
• Protein ID: WP_003816743.1
• Coordinates: 2093108..2093386 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR60_RS09955	2088538..2089632	+	1095	WP_003812637.1	TRAP transporter substrate-binding protein	-
CWR60_RS09960	2089844..2090473	+	630	WP_010928293.1	TRAP transporter small permease subunit	-
CWR60_RS09965	2090476..2092143	+	1668	WP_010928294.1	TRAP transporter large permease subunit	-
CWR60_RS09970	2092158..2092814	+	657	WP_010928295.1	phosphoribosylanthranilate isomerase	-
CWR60_RS09980	2093108..2093386	-	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
CWR60_RS09985	2093373..2093639	-	267	WP_003812626.1	BrnT family toxin	Toxin
CWR60_RS09990	2093870..2095411	-	1542	WP_010928296.1	M81 family metallopeptidase	-
CWR60_RS09995	2095473..2096351	-	879	WP_041937387.1	tripartite tricarboxylate transporter substrate binding protein	-
CWR60_RS10000	2096748..2097380	+	633	WP_003812619.1	PAS domain-containing protein	-
CWR60_RS10005	2097491..2098201	+	711	WP_003812617.1	aquaporin Z	-
CWR60_RS10010	2098240..2098512	-	273	WP_003812615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T89715 WP_003812626.1 NZ_CP025072:c2093639-2093373 [Bordetella parapertussis]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T89715 NZ_CP025072:c2093639-2093373 [Bordetella parapertussis]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGTGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT89715 WP_003816743.1 NZ_CP025072:c2093386-2093108 [Bordetella parapertussis]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT89715 NZ_CP025072:c2093386-2093108 [Bordetella parapertussis]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Toxin

• AlphaFold DB: K0MH61

Antitoxin

• AlphaFold DB: K0MF02