Detailed information of TA system TA089676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 147841..148346
• Replicon: chromosome
• Accession: NZ_CP025055
• Organism: Pseudomonas aeruginosa strain PB350

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: CWI21_RS00650
• Protein ID: WP_003083773.1
• Coordinates: 147841..148122 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: CWI21_RS00655
• Protein ID: WP_003083775.1
• Coordinates: 148119..148346 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI21_RS00625	143092..144441	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
CWI21_RS00630	144490..145176	+	687	WP_003083762.1	FadR family transcriptional regulator	-
CWI21_RS00635	145277..146011	+	735	WP_003083764.1	GntR family transcriptional regulator	-
CWI21_RS00640	146215..146601	+	387	WP_003083767.1	aegerolysin family protein	-
CWI21_RS00645	146633..147541	-	909	WP_003083769.1	LysR family transcriptional regulator	-
CWI21_RS00650	147841..148122	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
CWI21_RS00655	148119..148346	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
CWI21_RS00660	148522..149142	-	621	WP_003101226.1	hypothetical protein	-
CWI21_RS00665	149243..149743	+	501	WP_003083778.1	LEA type 2 family protein	-
CWI21_RS00670	149816..150157	+	342	WP_014603467.1	alkylphosphonate utilization protein	-
CWI21_RS00675	150239..151666	-	1428	WP_003083784.1	GABA permease	-
CWI21_RS00680	151835..153328	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T89676 WP_003083773.1 NZ_CP025055:c148122-147841 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T89676 NZ_CP025055:c148122-147841 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT89676 WP_003083775.1 NZ_CP025055:c148346-148119 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT89676 NZ_CP025055:c148346-148119 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGATGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9