Detailed information of TA system TA089670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 624282..624919
• Replicon: chromosome
• Accession: NZ_CP025053
• Organism: Pseudomonas aeruginosa strain PB354

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: CWI22_RS03030
• Protein ID: WP_019725766.1
• Coordinates: 624282..624464 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CWI22_RS03035
• Protein ID: WP_019725767.1
• Coordinates: 624497..624919 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI22_RS03015	621698..622069	-	372	WP_003142777.1	NERD domain-containing protein	-
CWI22_RS03020	622162..623283	-	1122	WP_033876353.1	Fic family protein	-
CWI22_RS03025	623542..623679	-	138	WP_003113217.1	hypothetical protein	-
CWI22_RS03030	624282..624464	+	183	WP_019725766.1	type II toxin-antitoxin system HicA family toxin	Toxin
CWI22_RS03035	624497..624919	+	423	WP_019725767.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CWI22_RS03040	625165..626136	-	972	WP_031672615.1	hypothetical protein	-
CWI22_RS03045	626121..626813	-	693	WP_023126965.1	hypothetical protein	-
CWI22_RS03050	626810..627352	-	543	WP_031672617.1	hypothetical protein	-
CWI22_RS03055	627349..628272	-	924	WP_100882537.1	hypothetical protein	-
CWI22_RS03060	628269..628745	-	477	WP_019725773.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	622162..676592	54430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-62)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6970.07 Da
• Isoelectric Point: 10.2954

>T89670 WP_019725766.1 NZ_CP025053:624282-624464 [Pseudomonas aeruginosa]
MKYSEFRRWLKARGVIFEPAKGSHFKVYYGDNQTIFPDHGAKEIGDGLRKKIIKDLGLKD

Nucleotide

• Length: 183 bp

>T89670 NZ_CP025053:624282-624464 [Pseudomonas aeruginosa]
ATGAAGTACAGCGAGTTTCGGCGCTGGTTGAAGGCTCGGGGAGTCATTTTCGAGCCTGCCAAGGGAAGCCATTTCAAGGT
TTACTACGGCGACAACCAGACGATCTTCCCCGACCATGGAGCCAAGGAAATAGGCGATGGGTTGAGGAAGAAGATAATCA
AAGACCTGGGCCTCAAGGATTGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 14970.22 Da
• Isoelectric Point: 4.9047

>AT89670 WP_019725767.1 NZ_CP025053:624497-624919 [Pseudomonas aeruginosa]
MFDYPVTVHEEAGSVWVSCDDVPEMASAGDTVDEALLDAVEGLESALSLYVDRRQSIPLPSKGKAGQSIVRLPALTSAKI
ALWNTMLAQNVGKAELARRLGVNRVQVDRLVDLLHGSKIEAVEHALAILGQRLAVTVIAA

Nucleotide

• Length: 423 bp

>AT89670 NZ_CP025053:624497-624919 [Pseudomonas aeruginosa]
ATGTTTGACTATCCCGTAACGGTTCACGAGGAAGCCGGCAGCGTGTGGGTCAGCTGCGACGATGTACCCGAAATGGCGAG
CGCCGGAGATACCGTTGATGAAGCGCTGCTGGACGCTGTGGAAGGCCTAGAGTCGGCCCTGTCGCTCTATGTGGACCGGC
GGCAGTCGATCCCACTGCCGTCGAAAGGCAAAGCGGGTCAGTCGATCGTTCGATTGCCCGCGCTCACTTCGGCCAAGATT
GCGCTGTGGAACACCATGCTGGCTCAGAACGTAGGGAAGGCGGAACTGGCTCGCCGGCTGGGAGTGAATCGCGTTCAGGT
AGACCGCCTGGTGGATCTGCTCCACGGGTCGAAGATCGAAGCGGTGGAACATGCTCTGGCGATCTTGGGCCAACGGTTGG
CTGTGACTGTCATTGCGGCATAA