Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4371126..4371734 Replicon chromosome
Accession NZ_CP025050
Organism Pseudomonas aeruginosa strain PB368

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag CWI24_RS21375 Protein ID WP_019486378.1
Coordinates 4371126..4371473 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag CWI24_RS21380 Protein ID WP_003114155.1
Coordinates 4371483..4371734 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWI24_RS21345 4366485..4366757 + 273 WP_073656953.1 cysteine-rich CWC family protein -
CWI24_RS21350 4366757..4367449 + 693 WP_022579823.1 16S rRNA pseudouridine(516) synthase -
CWI24_RS21355 4367585..4368628 + 1044 WP_003110811.1 L,D-transpeptidase -
CWI24_RS21360 4368708..4369445 + 738 WP_003085453.1 murein L,D-transpeptidase catalytic domain family protein -
CWI24_RS21365 4369897..4370799 + 903 WP_003085447.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
CWI24_RS21375 4371126..4371473 - 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CWI24_RS21380 4371483..4371734 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CWI24_RS21385 4371948..4372931 - 984 WP_003114154.1 tyrosine-type recombinase/integrase -
CWI24_RS21390 4372931..4374223 - 1293 WP_003115206.1 hypothetical protein -
CWI24_RS21395 4374453..4375736 - 1284 WP_023124144.1 zonular occludens toxin family protein -
CWI24_RS21400 4375740..4376096 - 357 WP_003114150.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4369897..4390638 20741


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T89659 WP_019486378.1 NZ_CP025050:c4371473-4371126 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T89659 NZ_CP025050:c4371473-4371126 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCTGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGTGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT89659 WP_003114155.1 NZ_CP025050:c4371734-4371483 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT89659 NZ_CP025050:c4371734-4371483 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References