Detailed information of TA system TA089656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2422644..2423686
• Replicon: chromosome
• Accession: NZ_CP025050
• Organism: Pseudomonas aeruginosa strain PB368

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CWI24_RS11750
• Protein ID: WP_003109777.1
• Coordinates: 2423111..2423686 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: CWI24_RS11745
• Protein ID: WP_003050245.1
• Coordinates: 2422644..2423114 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI24_RS11710	2418036..2419454	-	1419	WP_003109776.1	TIGR03752 family integrating conjugative element protein	-
CWI24_RS11715	2419444..2420355	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
CWI24_RS11720	2420352..2421044	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
CWI24_RS11725	2421041..2421439	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
CWI24_RS11730	2421451..2421810	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
CWI24_RS11735	2421827..2422060	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
CWI24_RS11740	2422057..2422440	-	384	WP_003105635.1	RAQPRD family integrative conjugative element protein	-
CWI24_RS11745	2422644..2423114	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
CWI24_RS11750	2423111..2423686	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
CWI24_RS11755	2423704..2424618	+	915	WP_043084563.1	AAA family ATPase	-
CWI24_RS11760	2424615..2425085	+	471	WP_003105626.1	hypothetical protein	-
CWI24_RS11765	2425082..2425582	+	501	WP_003090159.1	hypothetical protein	-
CWI24_RS11770	2425582..2426484	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
CWI24_RS11775	2426523..2427248	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T89656 WP_003109777.1 NZ_CP025050:2423111-2423686 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T89656 NZ_CP025050:2423111-2423686 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT89656 WP_003050245.1 NZ_CP025050:2422644-2423114 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT89656 NZ_CP025050:2422644-2423114 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68