89651

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 4338398..4339006 Replicon chromosome
Accession NZ_CP025049
Organism Pseudomonas aeruginosa strain PB369

Toxin (Protein)


Gene name PfiT Uniprot ID A0A444LUU5
Locus tag CWI25_RS21215 Protein ID WP_019486378.1
Coordinates 4338398..4338745 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag CWI25_RS21220 Protein ID WP_003114155.1
Coordinates 4338755..4339006 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWI25_RS21185 4333757..4334029 + 273 WP_073656953.1 cysteine-rich CWC family protein -
CWI25_RS21190 4334029..4334721 + 693 WP_022579823.1 16S rRNA pseudouridine(516) synthase -
CWI25_RS21195 4334857..4335900 + 1044 WP_003110811.1 L,D-transpeptidase -
CWI25_RS21200 4335980..4336717 + 738 WP_003085453.1 murein L,D-transpeptidase catalytic domain family protein -
CWI25_RS21205 4337169..4338071 + 903 WP_003085447.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
CWI25_RS21215 4338398..4338745 - 348 WP_019486378.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CWI25_RS21220 4338755..4339006 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CWI25_RS21225 4339220..4340203 - 984 WP_003114154.1 tyrosine-type recombinase/integrase -
CWI25_RS21230 4340203..4341495 - 1293 WP_003115206.1 hypothetical protein -
CWI25_RS21235 4341725..4343008 - 1284 WP_023124144.1 zonular occludens toxin family protein -
CWI25_RS21240 4343012..4343368 - 357 WP_003114150.1 DUF2523 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 4329323..4382337 53014


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12956.72 Da        Isoelectric Point: 4.4212

>T89651 WP_019486378.1 NZ_CP025049:c4338745-4338398 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T89651 NZ_CP025049:c4338745-4338398 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCTGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGTGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT89651 WP_003114155.1 NZ_CP025049:c4339006-4338755 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT89651 NZ_CP025049:c4339006-4338755 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A444LUU5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References