Detailed information of TA system TA089648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2385835..2386877
• Replicon: chromosome
• Accession: NZ_CP025049
• Organism: Pseudomonas aeruginosa strain PB369

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CWI25_RS11565
• Protein ID: WP_003109777.1
• Coordinates: 2386302..2386877 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: CWI25_RS11560
• Protein ID: WP_003050245.1
• Coordinates: 2385835..2386305 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI25_RS11525	2381227..2382645	-	1419	WP_003109776.1	TIGR03752 family integrating conjugative element protein	-
CWI25_RS11530	2382635..2383546	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
CWI25_RS11535	2383543..2384235	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
CWI25_RS11540	2384232..2384630	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
CWI25_RS11545	2384642..2385001	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
CWI25_RS11550	2385018..2385251	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
CWI25_RS11555	2385248..2385631	-	384	WP_003105635.1	RAQPRD family integrative conjugative element protein	-
CWI25_RS11560	2385835..2386305	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
CWI25_RS11565	2386302..2386877	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
CWI25_RS11570	2386895..2387809	+	915	WP_043084563.1	AAA family ATPase	-
CWI25_RS11575	2387806..2388276	+	471	WP_003105626.1	hypothetical protein	-
CWI25_RS11580	2388273..2388773	+	501	WP_003090159.1	hypothetical protein	-
CWI25_RS11585	2388773..2389675	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
CWI25_RS11590	2389714..2390439	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T89648 WP_003109777.1 NZ_CP025049:2386302-2386877 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T89648 NZ_CP025049:2386302-2386877 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT89648 WP_003050245.1 NZ_CP025049:2385835-2386305 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT89648 NZ_CP025049:2385835-2386305 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68