Detailed information of TA system TA089646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 151073..151578
• Replicon: chromosome
• Accession: NZ_CP025049
• Organism: Pseudomonas aeruginosa strain PB369

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: CWI25_RS00705
• Protein ID: WP_003083773.1
• Coordinates: 151073..151354 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: CWI25_RS00710
• Protein ID: WP_003083775.1
• Coordinates: 151351..151578 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI25_RS00680	146324..147673	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
CWI25_RS00685	147722..148402	+	681	WP_043084877.1	FadR family transcriptional regulator	-
CWI25_RS00690	148509..149243	+	735	WP_003083764.1	GntR family transcriptional regulator	-
CWI25_RS00695	149447..149833	+	387	WP_003083767.1	aegerolysin family protein	-
CWI25_RS00700	149865..150773	-	909	WP_003083769.1	LysR family transcriptional regulator	-
CWI25_RS00705	151073..151354	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
CWI25_RS00710	151351..151578	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
CWI25_RS00715	151754..152374	-	621	WP_003101226.1	hypothetical protein	-
CWI25_RS00720	152475..152975	+	501	WP_003162763.1	LEA type 2 family protein	-
CWI25_RS00725	153048..153389	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
CWI25_RS00730	153471..154898	-	1428	WP_003083784.1	GABA permease	-
CWI25_RS00735	155067..156560	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T89646 WP_003083773.1 NZ_CP025049:c151354-151073 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T89646 NZ_CP025049:c151354-151073 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGTAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT89646 WP_003083775.1 NZ_CP025049:c151578-151351 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT89646 NZ_CP025049:c151578-151351 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9