Detailed information of TA system TA089642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3201911..3202509
• Replicon: chromosome
• Accession: NZ_CP025048
• Organism: Escherichia coli strain SC516

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: CWI33_RS16085
• Protein ID: WP_053291281.1
• Coordinates: 3202132..3202509 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: CWI33_RS16080
• Protein ID: WP_001603498.1
• Coordinates: 3201911..3202132 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI33_RS16065	3197511..3198191	+	681	WP_053270475.1	fimbrial assembly chaperone	-
CWI33_RS16070	3198209..3200695	+	2487	WP_100881956.1	fimbria/pilus outer membrane usher protein	-
CWI33_RS16075	3200707..3201726	+	1020	WP_100881957.1	type 1 fimbrial protein	-
CWI33_RS16080	3201911..3202132	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CWI33_RS16085	3202132..3202509	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
CWI33_RS16095	3202677..3203954	-	1278	WP_011872831.1	ISL3 family transposase	-
CWI33_RS16100	3203967..3205001	-	1035	WP_089590277.1	permease	-
CWI33_RS16105	3205113..3205427	+	315	WP_000349485.1	helix-turn-helix domain-containing protein	-
CWI33_RS16110	3205612..3205827	-	216	Protein_2943	transposase	-
CWI33_RS16120	3206013..3207275	-	1263	WP_100882006.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3190099..3208230	18131

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T89642 WP_053291281.1 NZ_CP025048:3202132-3202509 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T89642 NZ_CP025048:3202132-3202509 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT89642 WP_001603498.1 NZ_CP025048:3201911-3202132 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT89642 NZ_CP025048:3201911-3202132 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0