Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2549253..2549865 Replicon chromosome
Accession NZ_CP025048
Organism Escherichia coli strain SC516

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag CWI33_RS12895 Protein ID WP_000833473.1
Coordinates 2549680..2549865 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A5F1W1Z0
Locus tag CWI33_RS12890 Protein ID WP_000499743.1
Coordinates 2549253..2549663 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWI33_RS12875 2544801..2545498 + 698 Protein_2374 IS1 family transposase -
CWI33_RS12880 2545806..2547206 + 1401 WP_000204832.1 MFS transporter -
CWI33_RS12885 2547217..2549181 + 1965 WP_040062336.1 glycoside hydrolase family 127 protein -
CWI33_RS12890 2549253..2549663 - 411 WP_000499743.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CWI33_RS12895 2549680..2549865 - 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
CWI33_RS12905 2550339..2551418 + 1080 WP_170854857.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
CWI33_RS12910 2551459..2552997 - 1539 WP_001359414.1 aldehyde dehydrogenase AldB -
CWI33_RS12915 2553162..2554313 - 1152 WP_000168699.1 L-threonine dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T89639 WP_000833473.1 NZ_CP025048:c2549865-2549680 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T89639 NZ_CP025048:c2549865-2549680 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15213.09 Da        Isoelectric Point: 4.5486

>AT89639 WP_000499743.1 NZ_CP025048:c2549663-2549253 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGA

Download         Length: 411 bp

>AT89639 NZ_CP025048:c2549663-2549253 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1W1Z0

References