Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 53923..54566 Replicon plasmid pNU-CRE047_2
Accession NZ_CP025039
Organism Klebsiella pneumoniae strain NU-CRE047

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CWQ24_RS29510 Protein ID WP_001044770.1
Coordinates 53923..54339 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CWQ24_RS29515 Protein ID WP_001261282.1
Coordinates 54336..54566 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWQ24_RS32655 49814..49879 + 66 Protein_52 EAL domain-containing protein -
CWQ24_RS29475 50019..50783 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
CWQ24_RS29485 50926..51192 - 267 WP_001144737.1 plasmid mobilization relaxosome protein MobC -
CWQ24_RS29490 51413..51886 - 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
CWQ24_RS29495 52042..52809 + 768 Protein_56 class 1 integron integrase IntI1 -
CWQ24_RS29500 52862..53566 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
CWQ24_RS29505 53613..53849 - 237 WP_001348075.1 AAA family ATPase -
CWQ24_RS29510 53923..54339 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CWQ24_RS29515 54336..54566 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CWQ24_RS29520 54523..54983 + 461 Protein_61 hypothetical protein -
CWQ24_RS32660 55111..55431 + 321 WP_032072060.1 hypothetical protein -
CWQ24_RS29525 55480..56118 + 639 WP_022644887.1 hypothetical protein -
CWQ24_RS29530 56121..56906 + 786 WP_022644886.1 site-specific integrase -
CWQ24_RS29535 56964..57221 - 258 WP_032408936.1 hypothetical protein -
CWQ24_RS32665 57350..57463 - 114 WP_032072094.1 hypothetical protein -
CWQ24_RS29545 58094..58849 + 756 WP_012539983.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..146689 146689


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T89581 WP_001044770.1 NZ_CP025039:c54339-53923 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T89581 NZ_CP025039:c54339-53923 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT89581 WP_001261282.1 NZ_CP025039:c54566-54336 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT89581 NZ_CP025039:c54566-54336 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References