Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 105764..106500 Replicon plasmid pNU-CRE047_1
Accession NZ_CP025038
Organism Klebsiella pneumoniae strain NU-CRE047

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag CWQ24_RS28555 Protein ID WP_003026803.1
Coordinates 105764..106246 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CWQ24_RS28560 Protein ID WP_003026799.1
Coordinates 106234..106500 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWQ24_RS28540 101401..102363 + 963 WP_004152113.1 M48 family metalloprotease -
CWQ24_RS28545 102451..103705 + 1255 Protein_98 IS3 family transposase -
CWQ24_RS28550 104206..105552 - 1347 WP_020314316.1 ISNCY family transposase -
CWQ24_RS28555 105764..106246 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
CWQ24_RS28560 106234..106500 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CWQ24_RS28565 106676..106930 - 255 WP_004152108.1 hypothetical protein -
CWQ24_RS28570 107006..107263 - 258 WP_004152107.1 hypothetical protein -
CWQ24_RS28575 107312..107515 - 204 WP_004152106.1 hemolysin expression modulator Hha -
CWQ24_RS28580 107549..107917 - 369 WP_004152105.1 hypothetical protein -
CWQ24_RS28585 107961..108455 - 495 WP_004152104.1 DNA-binding protein -
CWQ24_RS28590 108486..109061 - 576 WP_004152103.1 hypothetical protein -
CWQ24_RS28595 109049..109318 - 270 WP_004152102.1 hypothetical protein -
CWQ24_RS28600 109676..110026 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
CWQ24_RS28605 110076..110438 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / aadA2 / dfrA12 / catA1 - 1..199686 199686


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T89580 WP_003026803.1 NZ_CP025038:c106246-105764 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T89580 NZ_CP025038:c106246-105764 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT89580 WP_003026799.1 NZ_CP025038:c106500-106234 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT89580 NZ_CP025038:c106500-106234 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References