Detailed information of TA system TA089553

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3431665..3432322
• Replicon: chromosome
• Accession: NZ_CP025034
• Organism: Enterobacter sp. SGAir0187

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: W7P4L3
• Locus tag: CWR52_RS16570
• Protein ID: WP_023326024.1
• Coordinates: 3431912..3432322 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W7NX65
• Locus tag: CWR52_RS16565
• Protein ID: WP_006178375.1
• Coordinates: 3431665..3431931 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR52_RS16545	3427099..3428532	-	1434	WP_048029113.1	6-phospho-beta-glucosidase BglA	-
CWR52_RS16550	3428649..3429380	-	732	WP_104950163.1	MurR/RpiR family transcriptional regulator	-
CWR52_RS16555	3429646..3430305	+	660	WP_045335239.1	hemolysin III family protein	-
CWR52_RS16560	3430390..3431370	-	981	WP_104950164.1	tRNA-modifying protein YgfZ	-
CWR52_RS16565	3431665..3431931	+	267	WP_006178375.1	FAD assembly factor SdhE	Antitoxin
CWR52_RS16570	3431912..3432322	+	411	WP_023326024.1	protein YgfX	Toxin
CWR52_RS16575	3432329..3432850	-	522	WP_025757760.1	flavodoxin FldB	-
CWR52_RS16580	3432952..3433848	+	897	WP_008499709.1	site-specific tyrosine recombinase XerD	-
CWR52_RS16585	3433877..3434590	+	714	WP_104950165.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
CWR52_RS16590	3434596..3436329	+	1734	WP_104950166.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16063.06 Da
• Isoelectric Point: 10.9468

>T89553 WP_023326024.1 NZ_CP025034:3431912-3432322 [Enterobacter sp. SGAir0187]
VVLWQSDLRVSWRSQWMSLLLHGLVAAIVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDILGMPWMLNCGMMLRLRKVDGGRCQHLWLAADSMDAAEWRDLRRMLLQQTTQG

Nucleotide

• Length: 411 bp

>T89553 NZ_CP025034:3431912-3432322 [Enterobacter sp. SGAir0187]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTGTTGCTCCACGGGCTGGTTGCGGC
CATCGTTTTACTCATGCCGTGGCCGCTCAGCTATACGCCGCTGTGGCTGCTATTACTTTCGTTTGTTGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATCAAGTTACTGATGGATTCCCGCCTGCGCTGGCAGGGAAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTTTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTGTTGCAACAGACGA
CGCAGGGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10569.05 Da
• Isoelectric Point: 5.1340

>AT89553 WP_006178375.1 NZ_CP025034:3431665-3431931 [Enterobacter sp. SGAir0187]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDDDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT89553 NZ_CP025034:3431665-3431931 [Enterobacter sp. SGAir0187]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACACCTTAAGCGACGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: W7P4L3

Antitoxin

• AlphaFold DB: W7NX65