Detailed information of TA system TA089552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 2665228..2665844
• Replicon: chromosome
• Accession: NZ_CP025034
• Organism: Enterobacter sp. SGAir0187

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CWR52_RS12805
• Protein ID: WP_080347348.1
• Coordinates: 2665473..2665844 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2L2JED5
• Locus tag: CWR52_RS12800
• Protein ID: WP_048226827.1
• Coordinates: 2665228..2665470 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR52_RS12770	2660287..2661087	+	801	WP_104949838.1	DeoR/GlpR family DNA-binding transcription regulator	-
CWR52_RS12775	2661206..2661805	+	600	WP_104949839.1	glucose-1-phosphatase	-
CWR52_RS12780	2661799..2662680	+	882	WP_045329499.1	virulence factor BrkB family protein	-
CWR52_RS12785	2662677..2663114	+	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CWR52_RS12790	2663159..2664100	+	942	WP_161566068.1	fatty acid biosynthesis protein FabY	-
CWR52_RS12795	2664109..2665029	-	921	WP_104949841.1	alpha/beta hydrolase	-
CWR52_RS12800	2665228..2665470	+	243	WP_048226827.1	ribbon-helix-helix protein, CopG family	Antitoxin
CWR52_RS12805	2665473..2665844	+	372	WP_080347348.1	type II toxin-antitoxin system VapC family toxin	Toxin
CWR52_RS12810	2665884..2666813	-	930	WP_008501833.1	formate dehydrogenase accessory protein FdhE	-
CWR52_RS12815	2666810..2667445	-	636	WP_008501832.1	formate dehydrogenase cytochrome b556 subunit	-
CWR52_RS12820	2667442..2668344	-	903	WP_008501831.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13670.83 Da
• Isoelectric Point: 6.9790

>T89552 WP_080347348.1 NZ_CP025034:2665473-2665844 [Enterobacter sp. SGAir0187]
MEHMAVFDTNILIDLFNNRVEAANTIENTASHRSISLITWMEVMVGARKHGHEAKTAAVMGAFEVIDVTREIAERSVVLR
EKHGMKLPDAIILATAQSRNCPLITRNTKDFQGIAGVVLPYQL

Nucleotide

• Length: 372 bp

>T89552 NZ_CP025034:2665473-2665844 [Enterobacter sp. SGAir0187]
ATGGAACACATGGCGGTTTTTGATACCAATATCTTGATCGACCTTTTCAACAATCGCGTAGAGGCAGCAAATACGATCGA
AAACACAGCATCGCACCGGTCAATCAGTCTAATCACATGGATGGAAGTCATGGTAGGGGCGCGCAAACATGGCCACGAAG
CGAAAACCGCGGCGGTAATGGGCGCATTTGAGGTTATCGATGTAACCCGAGAGATCGCAGAAAGAAGCGTCGTGCTCCGT
GAGAAGCATGGCATGAAACTGCCGGATGCCATTATTTTAGCGACTGCCCAAAGCAGAAATTGCCCGTTAATCACCCGGAA
TACCAAAGACTTTCAGGGGATTGCCGGGGTGGTTTTACCTTATCAATTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9413.61 Da
• Isoelectric Point: 4.2584

>AT89552 WP_048226827.1 NZ_CP025034:2665228-2665470 [Enterobacter sp. SGAir0187]
MSLLAELSMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT89552 NZ_CP025034:2665228-2665470 [Enterobacter sp. SGAir0187]
ATGAGTTTACTGGCGGAGCTTAGCATGGGCAGAATTCTGATCGATTTGTCGGATGACGTTATTCAACGTCTCGATAACCT
CAAACAGCTGCGTAACCAACCTCGTGCCGAATTACTGCGTGAAGCAATTGAGCAGTATCTCGATCAGCAAAGCTCGTCCG
TTATCCGTGATGCGCTTGGTCTTTGGGGGAACCAGCAAGAAGATGGTTTAGAGTATGAGCGCAAGCTGCGTGAGGAGTGG
TAG

Structures

Antitoxin

• AlphaFold DB: A0A2L2JED5