Detailed information of TA system TA089549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 839221..840018
• Replicon: chromosome
• Accession: NZ_CP025034
• Organism: Enterobacter sp. SGAir0187

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: CWR52_RS04115
• Protein ID: WP_104948908.1
• Coordinates: 839221..839742 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: CWR52_RS04120
• Protein ID: WP_008499829.1
• Coordinates: 839749..840018 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR52_RS04085	834804..835493	+	690	WP_104948903.1	pyrimidine utilization protein B	-
CWR52_RS04090	835505..835891	+	387	WP_104948904.1	pyrimidine utilization protein C	-
CWR52_RS04095	835899..836699	+	801	WP_104948905.1	pyrimidine utilization protein D	-
CWR52_RS04100	836709..837299	+	591	WP_104948906.1	malonic semialdehyde reductase	-
CWR52_RS04105	837309..837803	+	495	WP_023327078.1	pyrimidine utilization flavin reductase protein F	-
CWR52_RS04110	837825..839147	+	1323	WP_104948907.1	pyrimidine utilization transport protein G	-
CWR52_RS04115	839221..839742	-	522	WP_104948908.1	GNAT family N-acetyltransferase	Toxin
CWR52_RS04120	839749..840018	-	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
CWR52_RS04125	840080..840997	-	918	WP_104948909.1	DMT family transporter	-
CWR52_RS04130	841115..841285	-	171	WP_023311077.1	general stress protein	-
CWR52_RS04135	841677..842273	+	597	WP_006809323.1	NAD(P)H:quinone oxidoreductase	-
CWR52_RS04140	842294..842521	+	228	WP_008499832.1	YccJ family protein	-
CWR52_RS04145	842555..843796	-	1242	WP_104948910.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
CWR52_RS04150	843949..844392	+	444	WP_045908316.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19855.64 Da
• Isoelectric Point: 8.9505

>T89549 WP_104948908.1 NZ_CP025034:c839742-839221 [Enterobacter sp. SGAir0187]
VENLTVEMFSEGKDYDFRDFDCGEPSLNVFLTEHLVRQHNGRILRGYLLKERDRPRVQGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGQEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDRAKRFYLKQGFIPLTGENSHSLF
FPTKSIERLFEQA

Nucleotide

• Length: 522 bp

>T89549 NZ_CP025034:c839742-839221 [Enterobacter sp. SGAir0187]
GTGGAAAATCTCACGGTCGAAATGTTTTCAGAGGGAAAAGATTATGATTTCAGGGATTTCGATTGTGGCGAACCCTCGCT
AAATGTCTTCCTCACAGAACATCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGGCTATCTGCTTAAAGAGCGCGATC
GTCCGCGAGTCCAGGGCTATTACACGTTGTCTGGGAGCTGTTTTGAAAAAGCGATGCTGCCTTCTAAGACTCAGCAGCGT
CGGATCCCTTATAGCAACGTACCGAGCGTAACGCTGGGACGTCTGGCGATACATAAAGACCTGCAGGGACAGGAGTGGGG
AACGACGCTCGTCACGCATGCCATGCGGGTTGTCTATCTGGCATCGCAGGCGGTGGGCGTACACGGAATATTTGTTGATG
CACTCAATGACCGGGCAAAACGATTTTATCTGAAGCAGGGTTTTATTCCGCTGACGGGTGAAAACAGCCATTCTCTTTTC
TTTCCTACAAAATCTATCGAACGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT89549 WP_008499829.1 NZ_CP025034:c840018-839749 [Enterobacter sp. SGAir0187]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT89549 NZ_CP025034:c840018-839749 [Enterobacter sp. SGAir0187]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGCGCTGCGGAGGTCATCGAACAGCATCGCCGTC
TCGTCTTAAACGAAGCATCATGGAATGCGGTGATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCAAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: W7PG43