Detailed information of TA system TA089546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 2461498..2462269
• Replicon: chromosome
• Accession: NZ_CP025031
• Organism: Staphylococcus haemolyticus strain SGAir0252

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: CWR44_RS12030
• Protein ID: WP_011275410.1
• Coordinates: 2461498..2461647 (-)
• Length: 50 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: Q4L7F8
• Locus tag: CWR44_RS12035
• Protein ID: WP_011275409.1
• Coordinates: 2461670..2462269 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR44_RS12010	2457113..2458222	-	1110	WP_011275414.1	GAF domain-containing sensor histidine kinase	-
CWR44_RS12015	2458410..2459234	+	825	WP_011275413.1	RluA family pseudouridine synthase	-
CWR44_RS12020	2459300..2460685	-	1386	WP_011275412.1	class II fumarate hydratase	-
CWR44_RS12025	2460892..2461287	-	396	WP_011275411.1	hypothetical protein	-
CWR44_RS12030	2461498..2461647	-	150	WP_011275410.1	hypothetical protein	Toxin
CWR44_RS12035	2461670..2462269	-	600	WP_011275409.1	glucosamine-6-phosphate isomerase	Antitoxin
CWR44_RS12040	2462441..2462911	-	471	WP_016931343.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
CWR44_RS12045	2462912..2464039	-	1128	WP_011275407.1	tRNA epoxyqueuosine(34) reductase QueG	-
CWR44_RS12050	2464217..2464939	-	723	WP_016931345.1	amino acid ABC transporter ATP-binding protein	-
CWR44_RS12055	2464932..2466386	-	1455	WP_016931346.1	ABC transporter substrate-binding protein/permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (130-195)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5796.18 Da
• Isoelectric Point: 3.9484

>T89546 WP_011275410.1 NZ_CP025031:c2461647-2461498 [Staphylococcus haemolyticus]
MTKNKDSELNYHEEENAMVQDLDDLKTLGKEMEQISEENDEDKLNQSHE

Nucleotide

• Length: 150 bp

>T89546 NZ_CP025031:c2461647-2461498 [Staphylococcus haemolyticus]
ATGACTAAAAATAAAGATTCAGAATTAAATTATCATGAGGAAGAAAATGCGATGGTTCAAGATTTAGATGACTTGAAAAC
CTTGGGTAAAGAAATGGAACAAATTTCTGAAGAAAACGACGAGGACAAGTTGAATCAATCGCATGAGTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22272.12 Da
• Isoelectric Point: 4.7588

>AT89546 WP_011275409.1 NZ_CP025031:c2462269-2461670 [Staphylococcus haemolyticus]
MAMNFKVFNDVEHVAEYTADIIRKQFNNNPTTIAGIHLTKDAAPVLDELKKDVDHNAVDFSQVNILDYDDNRSYYEALGV
PASQIYPINLDDDAESLIDDKIKTKENKGKLILQVTSIDESGSLNVNVRQGLLKAREVVLVVTGANKREVVKKLYEENGK
SSFEPSDLKAHRMVTVVLDRAAAEGLPEDVKEYFTARFA

Nucleotide

• Length: 600 bp

>AT89546 NZ_CP025031:c2462269-2461670 [Staphylococcus haemolyticus]
ATGGCAATGAACTTTAAAGTATTTAATGATGTAGAACATGTAGCTGAGTACACAGCAGATATCATTAGAAAGCAATTTAA
TAATAACCCTACAACTATTGCGGGAATTCATTTAACAAAGGATGCTGCACCTGTTTTAGATGAGTTAAAGAAAGATGTAG
ATCATAATGCGGTTGATTTTAGTCAAGTAAATATCTTAGATTATGACGATAATCGCTCTTACTATGAAGCATTAGGTGTA
CCTGCTAGTCAAATTTATCCAATTAATCTTGACGATGATGCAGAATCTTTAATTGATGACAAAATTAAAACTAAAGAAAA
TAAAGGTAAATTAATTTTACAAGTTACTTCTATTGATGAATCAGGTAGTTTAAATGTAAATGTAAGACAAGGATTATTAA
AAGCGCGTGAAGTAGTATTAGTTGTGACTGGTGCTAATAAACGTGAAGTTGTTAAAAAACTTTATGAAGAAAATGGTAAA
TCTAGCTTTGAACCATCTGATTTAAAAGCTCATAGAATGGTAACAGTTGTTTTAGATAGAGCAGCTGCAGAAGGTTTACC
TGAGGATGTTAAAGAATATTTCACTGCTAGATTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2A1K9C2