Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsaAT/-
Location 1440592..1441060 Replicon chromosome
Accession NZ_CP025031
Organism Staphylococcus haemolyticus strain SGAir0252

Toxin (Protein)


Gene name TsaT Uniprot ID Q4L4Q4
Locus tag CWR44_RS07065 Protein ID WP_011276326.1
Coordinates 1440592..1440780 (-) Length 63 a.a.

Antitoxin (Protein)


Gene name TsaA Uniprot ID Q4L4Q5
Locus tag CWR44_RS07070 Protein ID WP_011276325.1
Coordinates 1440803..1441060 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWR44_RS07035 1435801..1436547 - 747 WP_011276332.1 poly-gamma-glutamate hydrolase family protein -
CWR44_RS07040 1436684..1437292 + 609 WP_011276331.1 GNAT family N-acetyltransferase -
CWR44_RS07045 1437361..1438107 - 747 WP_104948261.1 hypothetical protein -
CWR44_RS07050 1438152..1439324 - 1173 WP_000195429.1 IS256-like element IS256 family transposase -
CWR44_RS07055 1439609..1439845 + 237 WP_016931008.1 hypothetical protein -
CWR44_RS07060 1439893..1440474 - 582 WP_011276327.1 hypothetical protein -
CWR44_RS07065 1440592..1440780 - 189 WP_011276326.1 hypothetical protein Toxin
CWR44_RS07070 1440803..1441060 - 258 WP_011276325.1 hypothetical protein Antitoxin
CWR44_RS07075 1441611..1441814 + 204 WP_033079647.1 sterile alpha motif-like domain-containing protein -
CWR44_RS07080 1441806..1442048 - 243 WP_011276324.1 hypothetical protein -
CWR44_RS07085 1442276..1442884 + 609 WP_011276323.1 phosphoglycerate mutase family protein -
CWR44_RS07090 1443071..1443688 - 618 WP_011276322.1 LysE/ArgO family amino acid transporter -
CWR44_RS07095 1443820..1444311 - 492 WP_011276321.1 GNAT family N-acetyltransferase -
CWR44_RS07100 1444524..1445840 - 1317 WP_011275188.1 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside SCCmec aac(6')-aph(2'') / blaZ clpC / tufA / clpP 961943..1495954 534011
- flank IS/Tn - - 1438152..1439324 1172
- flank IS/Tn - - 1444524..1445840 1316


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(25-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-68)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7110.80 Da        Isoelectric Point: 9.6269

>T89545 WP_011276326.1 NZ_CP025031:c1440780-1440592 [Staphylococcus haemolyticus]
MSLHLTILLWLGIIFVIAASIILGLLLKSKKEERKESYLGFTVIFYIFGFALLIYVFIFGIL

Download         Length: 189 bp

>T89545 NZ_CP025031:c1440780-1440592 [Staphylococcus haemolyticus]
ATGAGTTTGCATTTAACTATATTGTTATGGCTTGGTATCATTTTTGTAATAGCAGCCTCAATTATATTGGGCTTATTACT
TAAATCTAAAAAAGAAGAACGTAAAGAATCATATTTAGGTTTTACCGTAATATTTTATATTTTTGGATTTGCTTTACTCA
TCTACGTATTCATATTTGGTATATTATAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9914.97 Da        Isoelectric Point: 4.0625

>AT89545 WP_011276325.1 NZ_CP025031:c1441060-1440803 [Staphylococcus haemolyticus]
MNNNKIFWINIFMTLLFLGFNIIVTYNADLDDFFWLIPGLTISGITIVLSLSTALICKNLVSEVIFLINIVMLLYYIYPM
VYTFF

Download         Length: 258 bp

>AT89545 NZ_CP025031:c1441060-1440803 [Staphylococcus haemolyticus]
ATGAATAATAATAAAATTTTTTGGATAAATATTTTTATGACGCTATTATTTTTGGGTTTCAATATCATTGTCACTTACAA
TGCAGACTTAGATGATTTCTTCTGGCTAATTCCAGGACTGACAATTAGTGGTATCACAATTGTACTAAGCTTAAGTACTG
CGCTCATTTGCAAGAACCTGGTTAGCGAAGTTATTTTTCTAATCAATATTGTTATGCTTTTATACTACATTTATCCTATG
GTATATACATTCTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A1KAB5

Antitoxin

Source ID Structure
AlphaFold DB A0A2A1KAX9

References